Alternative splicing of the LIM-homeodomain transcription factor Isl1 in the mouse retina

Molecular and Cellular Neurosciences
Irene E WhitneyBenjamin E Reese

Abstract

Islet-1 (Isl1) is a LIM-homeodomain (LIM-HD) transcription factor that functions in a combinatorial manner with other LIM-HD proteins to direct the differentiation of distinct cell types within the central nervous system and many other tissues. A study of pancreatic cell lines showed that Isl1 is alternatively spliced generating a second isoform, Isl1β, which is missing 23 amino acids within the C-terminal region. This study examines the expression of the canonical and alternative Isl1 transcripts across other tissues, in particular, within the retina, where Isl1 is required for the differentiation of multiple neuronal cell types. The alternative splicing of Isl1 is shown to occur in multiple tissues, but the relative abundance of Isl1α and Isl1β expression varies greatly across them. In most tissues, Isl1α is the more abundant transcript, but in others the transcripts are expressed equally, or the alternative splice variant is dominant. Within the retina, differential expression of the two Isl1 transcripts increases as a function of development, with dynamic changes in expression peaking at E16.5 and again at P10. At the cellular level, individual retinal ganglion cells vary in their expression, with a subset of small-to-mediu...Continue Reading

References

Oct 7, 1997·Molecular and Cellular Biology·L W Jurata, G N Gill
Feb 1, 2000·Trends in Genetics : TIG·O Hobert, H Westphal
Mar 8, 2000·Mechanisms of Development·I Bach
Oct 11, 2003·Biological Psychiatry·Christopher J Lee, Kris Irizarry
Apr 2, 2004·The EMBO Journal·Sachin Katyal, Roseline Godbout
Nov 24, 2004·The Journal of Comparative Neurology·Tudor Constantin Badea, Jeremy Nathans
Jul 19, 2005·The Journal of Comparative Neurology·Jee-Hyun KongRichard H Masland
Apr 22, 2006·Neuroscience·J CoombsL M Chalupa
May 8, 2007·The Journal of Comparative Neurology·Yasser ElshatoryLin Gan
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yasser ElshatoryLin Gan
May 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiuqian MuWilliam H Klein
Jan 27, 2009·Investigative Ophthalmology & Visual Science·Irene E WhitneyBenjamin E Reese
Oct 6, 2010·BMC Genomics·Federico ZambelliGraziano Pesole
Jul 5, 2011·Nucleic Acids Research·Jun WanJiang Qian
Oct 27, 2012·Investigative Ophthalmology & Visual Science·Mónika LakkViktória Dénes
Jan 29, 2014·The Journal of Cell Biology·Takatoshi IijimaPeter Scheiffele
Mar 26, 2014·Nature Communications·Uygar SümbülH Sebastian Seung
Jul 12, 2014·Chemical Society Reviews·Hong-Cai Joe Zhou, Susumu Kitagawa

❮ Previous
Next ❯

Citations

Jul 1, 2015·Experimental Eye Research·Ruth Bejarano-EscobarJavier Francisco-Morcillo
Sep 14, 2016·Pathology, Research and Practice·Piotr CzapiewskiWojciech Biernat
Mar 7, 2021·International Journal of Molecular Sciences·Izarbe Aísa-MarínGemma Marfany

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.