Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels

Oncogene
Filip RoudnickyMichael Detmar

Abstract

Thrombospondin-2 (TSP2) is an anti-angiogenic matricellular protein that inhibits tumor growth and angiogenesis. Tumor-associated blood vascular endothelial cells (BECs) were isolated from human invasive bladder cancers and from matched normal bladder tissue by immuno-laser capture microdissection. Exon expression profiling analyses revealed a particularly high expression of a short TSP2 transcript containing only the last 9 (3') exons of the full-length TSP2 transcript. Using 5' and 3' RACE (rapid amplification of cDNA ends) and Sanger sequencing, we confirmed the existence of the shorter transcript of TSP2 (sTSP2) and determined its sequence which completely lacked the anti-angiogenic thrombospondin type 1 repeats domain. The largest open reading frame predicted within the transcript comprises 209 amino acids and matches almost completely the C-terminal lectin domain of full-length TSP2. We produced recombinant sTSP2 and found that unlike the full-length TSP2, sTSP2 did not inhibit vascular endothelial growth factor-A-induced proliferation of cultured human BECs, but in contrast when combined with TSP2 blocked the inhibitory effects of TSP2 on BEC proliferation. In vivo studies with stably transfected A431 squamous cell carci...Continue Reading

References

Aug 12, 1971·The New England Journal of Medicine·J Folkman
Dec 5, 1995·Biochemical and Biophysical Research Communications·O V VolpertN Bouck
Feb 1, 1997·The Journal of Laboratory and Clinical Medicine·T S PanettiD F Mosher
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D HanahanJ Arbeit
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·T R KyriakidesP Bornstein
Nov 26, 1999·The Journal of Investigative Dermatology·T R KyriakidesP Bornstein
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M StreitM Detmar
Jan 4, 2001·Journal of Dermatological Science·M Detmar
Jun 12, 2002·Molecular Biology of the Cell·Lucas C ArmstrongPaul Bornstein
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Jack Lawler, Michael Detmar
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Jun 2, 2005·Nature Reviews. Cancer·Dario Neri, Roy Bicknell
Jun 18, 2005·Bioinformatics·Melissa S ClineAlan Williams
Nov 30, 2005·Methods : a Companion to Methods in Enzymology·Karpagam SrinivasanManuel Ares
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jul 25, 2006·Molecular & Cellular Proteomics : MCP·Vincent CastronovoDario Neri
Mar 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ronald J BuckanovichGeorge Coukos
Jan 15, 2008·Cellular and Molecular Life Sciences : CMLS·C B CarlsonD F Mosher
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Mar 24, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jingfang SunLaura E Benjamin
Apr 16, 2009·Cancer Research·Bin RenRoya Khosravi-Far
Dec 10, 2009·Cancer Research·Beatrice BorgiaRaffaella Giavazzi
Jun 2, 2010·Nucleic Acids Research·Dorothea EmigMario Albrecht
Sep 15, 2010·The Journal of Experimental Medicine·Reto HuggenbergerMichael Detmar
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Patrick R Lawler, Jack Lawler
Sep 8, 2012·Nature·Sarah DjebaliThomas R Gingeras

❮ Previous
Next ❯

Citations

Mar 6, 2019·Bioscience Reports·Jin-Yan Wang, Li-Juan Chen
Jul 25, 2019·PloS One·Sun-Young YoonMichael Detmar
Dec 19, 2019·Frontiers in Genetics·Victor G MartinezJesus Maria Paramio
Jul 13, 2021·Frontiers in Oncology·Farshad Moradi KashkooliArman Rahmim

❮ Previous
Next ❯

Datasets Mentioned

BETA
BX641023
GSE41614

Methods Mentioned

BETA
reverse transcription PCR
PCR
biopsies
Cytomation
chip
transfection
column affinity purification
protein
electrophoresis

Software Mentioned

MIDAS
limma
Bioconductor
Genespring Gx
ANOSVA
affymetrix
GraphPad
Partek Genomics Suite
R
FANTOM5

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer Disparities

Cancer disparities refers to differences in cancer outcomes (e.g., number of cancer cases, related health complications) across population groups.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.