Alternative translation initiation codon for the human melanocortin MC3 receptor does not affect the ligand binding

European Journal of Pharmacology
H B SchiöthM Szardenings

Abstract

The genomic DNA for the human melanocortin MC3 receptor indicates an unusually long N-terminus. Two possible translation initiation sites, the one originally proposed and one alternate 111 bp downstream, were mutated. For a third mutant the DNA between these initiation sites was deleted. All mutants were expressed in COS (CV-1 Origin, SV40) cells in the same level, and they bound peptide hormones in the same fashion, as did the wild type clone. The data obtained indicate that both sites can function as the sole translation initiation sites of the human clone and that the proposed N-terminus of the human melanocortin MC3 receptor is not important for the ligand binding of the receptor.

References

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Feb 15, 1995·European Journal of Pharmacology·H B SchiöthV Chhajlani
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Citations

Aug 15, 1998·Molecular and Cellular Endocrinology·H B SchiöthJ E Wikberg
Jan 29, 2016·Nature Communications·Bonggi LeeJack A Yanovski
Aug 6, 2002·Diabetes Research and Clinical Practice·Jencia WongKathleen G Mountjoy
Nov 5, 1997·The Journal of Biological Chemistry·M SzardeningsJ E Wikberg
May 19, 2000·Annals of the New York Academy of Sciences·S Solomon
Jun 30, 1997·FEBS Letters·H B SchiöthJ E Wikberg

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