Alternative usage of exon 1 of bovine PrP mRNA

Biochemical and Biophysical Research Communications
M HoriuchiM Shinagawa

Abstract

Here we report two types of bovine prion protein (PrP) mRNA that possessed different lengths of the 5'-untranslated region and were expressed in various bovine tissues. The two mRNA species were transcribed from identical positions but differed in the usage of the splice site for exon 1/intron. One mRNA possessed exon 1 consisting of 53 nucleotides and the other possessed exon 1 consisting of 168 nucleotides. Usage of exons 2 and 3 was identical for the two mRNA species. The two mRNA species were detected in all but spleen tissue; the mRNA possessing 168-nt exon 1 was not detected in bovine spleen. This is the first report on the tissue-specific alternative splicing of PrPc mRNA in any other species. Only a low level of PrPc appeared to be present in bovine spleen. These results suggested the possibility that the mRNA possessing 53-nt exon 1 was inefficiently translated into Prp; however, in vitro translation analysis showed no marked difference in translational efficiency between the two mRNA species.

References

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Citations

May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·C Lemaire-VieilleJ Y Cesbron
Mar 23, 2007·Journal of Molecular Biology·Cathryn L HaighDavid R Brown
May 21, 2003·Animal Genetics·D HillsJ L Williams
May 1, 2008·The FEBS Journal·Yutaka KikuchiKosuke Takatori
Jan 23, 1999·European Journal of Biochemistry·S DugaT Simonic
Feb 21, 2008·Veterinary Research·Wilfred Goldmann
Sep 11, 2004·Viral Immunology·Nathalie Daude

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