Alternatively spliced FGFR-1 isoforms differentially modulate endothelial cell activation of c-YES

Archives of Biochemistry and Biophysics
Pei ZhangJohn A Thompson

Abstract

Ligand activation of fibroblast growth factor receptor-1 (FGFR-1) induces an angiogenic response following activation of multiple intracellular signaling substrates, including the Src family of nonreceptor tyrosine kinases (SFK). However, the direct association between FGFR-1 and SFK and the involvement of SFK in FGFR-1-dependent cell proliferation have been controversial. Structural variants of FGFR-1 are generated by alternative splicing which results in two major isoforms, containing either three (FGFR-1alpha) or two (FGFR-1beta) immunoglobulin-like domains in the extracellular region. To determine whether alternatively spliced FGFR-1 isoforms differentially activate SFK, we have examined FGF receptor-negative endothelial cells stably transfected with human cDNA encoding either FGFR-1alpha or FGFR-1beta. Transient activation of c-YES, the predominant SFK expressed in these endothelial cells, was restricted to FGFR-1beta transfectants following exposure to acidic fibroblast growth factor (FGF-1). Co-immunoprecipitation studies revealed that c-YES directly associated with FGFR-1beta. The Src homology (SH)2 domain (and not the SH3 domain) of c-YES was able to recognize tyrosine phosphorylated FGFR-1beta. FGFR-1beta-specific act...Continue Reading

References

Sep 9, 1988·Science·J A ThompsonT Maciag
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A ThompsonT Maciag
Jan 1, 1989·Annual Review of Biochemistry·W H Burgess, T Maciag
Jul 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R E Friesel, T Maciag
Jul 21, 1995·The Journal of Biological Chemistry·S R OpalenikJ A Thompson
Dec 1, 1993·Molecular and Cellular Biology·Y ZhaoH Hanafusa
Jan 1, 1993·Advances in Cancer Research·D E Johnson, L T Williams
Apr 1, 1996·Archives of Otolaryngology--head & Neck Surgery·M A WalterJ A Thompson
Jun 21, 1996·The Journal of Biological Chemistry·D M OrnitzM Goldfarb
Jun 5, 1996·Biochimica Et Biophysica Acta·J T ShinJ A Thompson
Jul 1, 1997·The Journal of Investigative Dermatology·H TakenakaH Yasuno
Dec 2, 1999·Experimental Cell Research·K A DeMaliA Kazlauskas
Jan 7, 2000·Experimental Cell Research·C L Abram, S A Courtneidge
Mar 29, 2001·Genome Biology·D M Ornitz, N Itoh
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ShimadaT Yamashita
Jun 22, 2001·Experimental and Molecular Pathology·M MarxJ A Madri
Jul 20, 2002·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Selwyn M VickersJohn A Thompson
Feb 8, 2003·Archives of Biochemistry and Biophysics·Jing JiaoJohn A Thompson
Feb 25, 2003·The Journal of Biological Chemistry·Deena SmallThomas Maciag
Mar 7, 2003·The Journal of Biological Chemistry·Dawn M KilkennyGeraldine G Miller
Aug 20, 2003·Development·Bruce W DraperCharles B Kimmel

❮ Previous
Next ❯

Citations

Oct 1, 2013·International Journal of Cell Biology·Zahava SiegfriedRotem Karni
May 16, 2014·Journal of Signal Transduction·Rajesh RajuHarsha Gowda
Apr 16, 2014·Journal of Cellular Physiology·Siew-Ging Gong
Apr 9, 2013·Current Opinion in Structural Biology·Sara Light, Arne Elofsson
Aug 23, 2012·Gene·Olga KelemenStefan Stamm
Oct 22, 2011·Oral Oncology·Marcia S CamposJacques E Nör
Dec 30, 2014·Fundamental & Clinical Pharmacology·Amit KumarNirmal Singh
Dec 29, 2011·Journal of Nucleic Acids·Klaus HolzmannBrigitte Marian
Mar 24, 2015·Frontiers in Endocrinology·Marjut PihlajokiDavid B Wilson
Feb 18, 2017·International Journal of Molecular Sciences·Cherine Abou-FayçalBeatrice Eymin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.