Alteromonas Myovirus V22 Represents a New Genus of Marine Bacteriophages Requiring a Tail Fiber Chaperone for Host Recognition

MSystems
Rafael Gonzalez-SerranoFrancisco Rodriguez-Valera

Abstract

Marine phages play a variety of critical roles in regulating the microbial composition of our oceans. Despite constituting the majority of genetic diversity within these environments, there are relatively few isolates with complete genome sequences or in-depth analyses of their host interaction mechanisms, such as characterization of their receptor binding proteins (RBPs). Here, we present the 92,760-bp genome of the Alteromonas-targeting phage V22. Genomic and morphological analyses identify V22 as a myovirus; however, due to a lack of sequence similarity to any other known myoviruses, we propose that V22 be classified as the type phage of a new Myoalterovirus genus within the Myoviridae family. V22 shows gene homology and synteny with two different subfamilies of phages infecting enterobacteria, specifically within the structural region of its genome. To improve our understanding of the V22 adsorption process, we identified putative RBPs (gp23, gp24, and gp26) and tested their ability to decorate the V22 propagation strain, Alteromonas mediterranea PT11, as recombinant green fluorescent protein (GFP)-tagged constructs. Only GFP-gp26 was capable of bacterial recognition and identified as the V22 RBP. Interestingly, production ...Continue Reading

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Datasets Mentioned

BETA
MG592441
MK562505
KY652726
MN877442

Methods Mentioned

BETA
transmission electron microscopy
fluorescence
pulldown
fluorescence microscopy
Fluorescence spectrometry
size-exclusion chromatography
restriction modification
pulldowns
PCR

Software Mentioned

ImageJ
MULTALIN
FastQC
HHpred
HIT
CD
tBLASTx
ModelFinder
MUSCLE
BLASTn

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