Altitudinal variation of berberine, total phenolics and flavonoid content in Thalictrum foliolosum and their correlation with antimicrobial and antioxidant activities

Journal of Ayurveda and Integrative Medicine
Garima PandeyA K S Rawat

Abstract

The quality of herbal medicine is determined by its secondary metabolites, which may vary according to growth, season and altitude etc. OBJECTIVE: We studied the variation in phytochemistry and biological activities of Thalictrum foliolosum (TF) roots collected from four sites at different altitudes. The berberine content in different extracts of T. foliolosum roots collected from various altitudes was estimated using HPTLC. Total phenolic and flavonoid contents were determined using Folin-Ciocalteau reagent and aluminum chloride method respectively. The sensitivity of microbes for the extracts was studied using disc diffusion and the MIC was estimated using broth dilution method. Antioxidant capacity of the plant was studied using β-carotene bleaching assay, lipid peroxidation assay using goat liver, reducing power assay and DPPH free radical scavenging activity. Berberine content varied inversely with altitude; while phenol and flavonoid content of TF increased at higher altitudes. All the TF extracts showed moderate to high activity against Candida albicans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Extracts with high berberine content were most effective against C. albicans and S. aureus and also s...Continue Reading

Citations

Nov 28, 2019·Journal of Asian Natural Products Research·Nan Sun, Yun Han
Sep 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna PetruczynikGrażyna Szymczak
Nov 14, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinmei XuGang Fan

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BETA
environmental stresses

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XLSTAT
winCATS

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