PMID: 8993978Jan 1, 1997Paper

Alu-polymerase chain reaction genomic fingerprinting technique identifies multiple genetic loci associated with pancreatic tumourigenesis

Genes, Chromosomes & Cancer
A B McKieN R Lemoine

Abstract

DNA fingerprinting can be used to detect genetic rearrangements in cancer that may be associated with activation of oncogenes and inactivation of tumour suppressor genes. We have developed a fingerprinting strategy based on polymerase chain reaction (PCR) amplification of genomic DNA with primers specific for the Alu repeat sequences, which are highly abundant in the human genome. This has been applied to DNA from pancreatic cancer and paired normal samples to isolate and identify fragments of genomic DNA rearranged in the malignant cells. These fragments have been sequenced and used as probes to isolate hybridising clones from gridded bacteriophage P1, phage artificial chromosome, and cosmid libraries for fluorescent in situ hybridisation mapping and the identification of expressed sequences. Further characterisation has identified a putative novel gene (ART1) that is up-regulated specifically in pancreatic cancer as well as another sequence with similarity to genes involved in differentiation (POU domains). In conclusion, we suggest that Alu-PCR fingerprinting may be a useful technique for the identification of genes involved in tumourigenesis.

References

Jan 1, 1992·Gastroenterology·N R LemoineG Klöppel
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M A PeinadoM Perucho
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C PierceN Sternberg
Oct 1, 1991·The Journal of Experimental Medicine·B TyckoJ Sklar
Dec 1, 1991·British Journal of Cancer·C M BartonR C Williamson
Oct 1, 1991·Japanese Journal of Cancer Research : Gann·A YanagisawaH Sugano
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Mar 11, 1991·Nucleic Acids Research·T A Holton, M W Graham
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Apr 1, 1988·Molecular and Cellular Biology·J SkowronskiM F Singer
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L NelsonC T Caskey
Mar 25, 1985·Nucleic Acids Research·P G SealeyE M Southern
Jun 4, 1982·Science·C W Schmid, W R Jelinek
Nov 11, 1983·Nucleic Acids Research·G R DanielsP L Deininger
Nov 1, 1995·Nature Medicine·M Nyström-LahtiB Vogelstein
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·M SchutteJ M Trent
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T M GressH Lehrach
Jul 1, 1994·International Journal of Cancer. Journal International Du Cancer·B JohanssonF Mitelman
Oct 21, 1993·International Journal of Cancer. Journal International Du Cancer·A B McKieN R Lemoine
Feb 12, 1993·Science·N LisitsynM Wigler
May 1, 1993·British Journal of Cancer·G BardiS Heim
Nov 1, 1995·Genes, Chromosomes & Cancer·D BartschP J Goodfellow
Nov 1, 1995·Cancer·J E NiederhuberH R Menck

❮ Previous
Next ❯

Citations

May 20, 2004·Annals of Surgical Oncology·SuEllen Toth-FejelRodney Pommier
May 25, 2005·International Journal of Cancer. Journal International Du Cancer·Tapasya SrivastavaSubrata Sinha

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.