Aluminum detoxication mechanism in Pseudomonas fluorescens is dependent on iron

FEMS Microbiology Letters
V D Appanna, Robert Hamel

Abstract

The soil microbe Pseudomonas fluorescens has been shown to detoxify aluminum by the elaboration of a soluble metabolite where the trivalent metal is sequestered [Appanna and St. Pierre, FEMS Microbiol. Lett. 24 (1994) 327-332]. The inclusion of 5 mM iron in the growth medium elicited an entirely disparate detoxification strategy. In this instance, the two trivalent metals were immobilized in a gelatinous lipid-rich residue. Dialysis and ultracentrifugation studies indicated that the test metals were being transformed from early stages of growth and were associated with phosphatidylethanolamine. However, at 45 h of cellular multiplication, most of the metals were deposited as an insoluble residue. X-ray fluorescence analyses identified the constituents of this mineral essentially as aluminum, iron and phosphorus. Scanning electron microscopy and energy dispersive X-ray microanalysis of the dialysate, isolated at 35 h of microbial growth, revealed thread-like structures associated with nodule-like bodies that were rich in the two test metals. Transmission electron microscopic studies aided in the visualization of iron and aluminum inclusions within the bacterial cells.

References

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Citations

Aug 10, 2000·FEMS Microbiology Letters·F KawaiM Sugimoto
Mar 23, 2013·Environmental Monitoring and Assessment·Samina WasiMasood Ahmad
Dec 17, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Christopher Exley, Matthew J Mold
Jun 20, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Mohd Wajid Ali Khan, Masood Ahmad

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