Aluminum doping tunes band gap energy level as well as oxidative stress-mediated cytotoxicity of ZnO nanoparticles in MCF-7 cells

Scientific Reports
Mohd Javed AkhtarMaqusood Ahamed

Abstract

We investigated whether Aluminum (Al) doping tunes band gap energy level as well as selective cytotoxicity of ZnO nanoparticles in human breast cancer cells (MCF-7). Pure and Al-doped ZnO nanoparticles were prepared by a simple sol-gel method. Characterization study confirmed the formation of single phase of Al(x)Zn(1-x)O nanocrystals with the size range of 33-55 nm. Al-doping increased the band gap energy of ZnO nanoparticles (from 3.51 eV for pure to 3.87 eV for Al-doped ZnO). Al-doping also enhanced the cytotoxicity and oxidative stress response of ZnO nanoparticles in MCF-7 cells. The IC50 for undoped ZnO nanoparticles was 44 μg/ml while for the Al-doped ZnO counterparts was 31 μg/ml. Up-regulation of apoptotic genes (e.g. p53, bax/bcl2 ratio, caspase-3 &caspase-9) along with loss of mitochondrial membrane potential suggested that Al-doped ZnO nanoparticles induced apoptosis in MCF-7 cells through mitochondrial pathway. Importantly, Al-doping did not change the benign nature of ZnO nanoparticles towards normal cells suggesting that Al-doping improves the selective cytotoxicity of ZnO nanoparticles toward MCF-7 cells without affecting the normal cells. Our results indicated a novel approach through which the inherent selecti...Continue Reading

References

Nov 1, 1978·Journal of Clinical Pathology·A G Higginson, R L Pile
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Dec 1, 1994·British Journal of Cancer·Y KubotaA Fujishima
Jun 15, 1988·Physical Review. B, Condensed Matter·B E SerneliusC G Granqvist
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE
Sep 29, 2004·The Biochemical Journal·Pablo Fuentes-Prior, Guy S Salvesen
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Aug 30, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S M HussainJ J Schlager
Feb 4, 2006·Science·Andre NelNing Li
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Dec 13, 2006·Cancer Metastasis Reviews·Wen-Sheng Wu
Sep 18, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard C MurdockSaber M Hussain
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Richard J Youle, Andreas Strasser
May 24, 2008·Journal of the American Chemical Society·Huan-Ming XiongYong-Yao Xia
Sep 11, 2008·Journal of the National Cancer Institute·Quirine Lamberts OkonkwoHarry J de Koning
Apr 22, 2009·Toxicology Letters·Vyom SharmaAlok Dhawan
May 30, 2009·Nature Reviews. Drug Discovery·Dunyaporn TrachoothamPeng Huang
Feb 17, 2010·Chemical Research in Toxicology·Philip J MoosJohn M Veranth
Mar 4, 2010·Colloids and Surfaces. B, Biointerfaces·P GopinathSiddhartha Sankar Ghosh
Mar 20, 2010·European Journal of Medicinal Chemistry·Subban RaviSathees C Raghavan
Aug 19, 2010·Expert Opinion on Drug Delivery·John W RasmussenDenise G Wingett
Nov 3, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Mariappan PremanathanGovindasamy Manivannan
Jun 15, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Maqusood AhamedSalman A Alrokayan
Jan 17, 2012·Toxicology and Applied Pharmacology·Javed AhmadAbdulaziz A Al-Khedhairy
Jan 26, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Maqsood A SiddiquiSalman A Alrokayan
Mar 8, 2012·Apoptosis : an International Journal on Programmed Cell Death·Vyom SharmaAlok Dhawan
Mar 15, 2014·OncoTargets and Therapy·Maqusood Ahamed, Hisham A Alhadlaq

❮ Previous
Next ❯

Citations

Mar 16, 2017·Advanced Materials·Dongyoon KhimThomas D Anthopoulos
Feb 1, 2019·Advanced Materials·Liang YanYuliang Zhao
May 8, 2020·Environmental Science and Pollution Research International·Adriana Martinez-OviedoSoo Wohn Lee
Mar 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohd Javed AkhtarZabnAllah M Alaizeri
Jun 24, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·D Q TrungP T Huy
Jun 12, 2019·Accounts of Chemical Research·Joseph T BuchmanChristy L Haynes

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
PCR
X-ray
transmission electron microscopy
dynamic light scattering
fluorescence imaging

Software Mentioned

AlphaEase TM FC StandAlone
Prism
GraphPad

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.