Aluminum Elicits Exocellular Phosphatidylethanolamine Production in Pseudomonas fluorescens.

Applied and Environmental Microbiology
Vasu D Appanna, M S Pierre

Abstract

Pseudomonas fluorescens ATCC 13525 was found to grow in a minimal mineral medium supplemented with millimolar amounts of aluminum, a known environmental toxicant. During the stationary phase of growth, the trivalent metal was localized in a phosphatidylethanolamine (PE)-containing residue. The concentration of PE in pellets ranged from 1.7 to 13.9 mg ml of culture(sup-1) in media supplemented with 1 to 30 mM aluminum. Although the gelatinous residue was observed during the stationary phase of growth, ultracentrifugation and dialysis experiments revealed that PE was produced from earlier stages of incubation and was associated with aluminum. A sharp diminution in the levels of PE and aluminum in the spent fluid was concomitant with the formation of the insoluble deposit. The aluminum content of the soluble cellular fraction increased during growth and reached an optimum of 1.85 mM of test metal at 45 h in cultures with 15 mM aluminum. Further incubation, however, led to a marked decrease in the cellular aluminum content, and during the stationary phase of growth, only trace amounts of the trivalent metal were detected in this fraction. When 45-h cells were incubated in fresh citrate medium, most of the intracellular aluminum was...Continue Reading

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Citations

Aug 23, 2008·Journal of Basic Microbiology·Joseph LemireVasu D Appanna
Jan 22, 2020·Journal of Medical Microbiology·Alex MacLeanVasu D Appanna
Sep 27, 2003·Environmental Microbiology·Mónica SantamaríaJavier Corzo
Jul 4, 2003·Applied and Environmental Microbiology·J E AmonetteJ T Ho
Nov 16, 2001·Journal of Inorganic Biochemistry·R D Hamel, V D Appanna
Dec 28, 1999·Journal of Inorganic Biochemistry·R HamelV D Appanna

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