Aluminum induces rapidly mitochondria-dependent programmed cell death in Al-sensitive peanut root tips

Botanical Studies
Wen-Jing HuangLong-Fei He

Abstract

Although many studies suggested that aluminum (Al) induced programmed cell death (PCD) in plants, the mechanism of Al-induced PCD and its effects in Al tolerance is limited. This study was to investigate the mechanism and type of Al induced PCD and the relationship between PCD and Al tolerance. In this study, two genotypes of peanut 99-1507 (Al tolerant) and ZH2 (Al sensitive) were used to investigate Al-induced PCD. Peanut root growth inhibition induced by AlCl3 was concentration and time-dependent in two peanut varieties. AlCl3 at 100 μM could induce rapidly peanut root tip PCD involved in DNA cleavage, typical apoptotic chromatin condensation staining with DAPI, apoptosis related gene Hrs203j expression and cytochrome C (Cyt c) release from mitochondria to cytosol. Caspase3-like protease was activated by Al; it was higher in ZH2 than in 99-1507. Al increased the opening of mitochondrial permeability transition pore (MPTP), decreased inner membrane potential (ΔΨm) of mitochondria. Compared with the control, Al stress increased O2(•-) and H2O2 production in mitochondria. Reactive oxygen species (ROS) burst was produced at Al treatment for 4 h. Al-induced PCD is earlier and faster in Al-sensitive peanut cultivar than in Al-tole...Continue Reading

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Citations

Jan 1, 2018·Functional Plant Biology : FPB·Vadim DemidchikOlga V Voitsekhovskaja

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Methods Mentioned

BETA
electrophoresis
PCR
Assay
Fluorescence microscopy
transgenics

Software Mentioned

Excel2007
SPSS12

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis