Alveolar macrophage inducible nitric oxide synthase-dependent pulmonary microvascular endothelial cell septic barrier dysfunction

Microvascular Research
K S FarleyS Mehta

Abstract

Inducible nitric oxide (NO) synthase (iNOS) from neutrophils and alveolar macrophages (AM) contributes to the pathophysiology of murine septic acute lung injury (ALI). It is not known if AM iNOS has a direct effect on septic pulmonary microvascular endothelial cell (PMVEC) permeability. We hypothesized that AM iNOS mediates PMVEC permeability in vitro under septic conditions through NO and peroxynitrite. 100,000 confluent PMVEC on cell-culture inserts were co-incubated with iNOS+/+ vs. iNOS-/- AM, in various ratios of AM to PMVEC. PMVEC injury was assessed by trans-PMVEC Evans Blue-labelled albumin flux in the presence or absence of cytomix (equimolar TNF-alpha, IL-1beta and IFN-gamma). Cytomix stimulation dose-dependently increased trans-PMVEC EB-albumin flux, which was exaggerated (1.4+/-0.1% vs. 0.4+/-0.1% in unstimulated PMVEC, p<0.05) in the presence of iNOS+/+, but not iNOS-/-, AM in the upper compartment. Similarly, iNOS+/+, but not iNOS-/-, AM in the lower compartment also enhanced septic trans-PMVEC albumin leak. The mechanism of iNOS-dependent septic PMVEC permeability was pursued through pharmacologic studies with inhibitors of NOS, and scavengers of NO, superoxide, and peroxynitrite, and treatment of PMVEC with the ...Continue Reading

References

Jan 1, 1995·Annual Review of Pharmacology and Toxicology·D L Laskin, K J Pendino
Feb 1, 1994·Archives of Surgery·E A MinnardJ M Daly
Nov 1, 1996·The Journal of Surgical Research·Y TsukaharaM Torisu
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·M J ThomassenM S Kavuru
Oct 23, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M J HickeyP Kubes
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Mar 25, 1998·The Journal of Antimicrobial Chemotherapy·J R Parratt
Jun 20, 1998·The Journal of Biological Chemistry·R J RohlfsR M Winslow
Jun 23, 1998·The Journal of Biological Chemistry·T P MiskoD Salvemini
Aug 26, 1998·The Journal of Biological Chemistry·Y XiaJ L Zweier
Sep 2, 1998·The Annals of Thoracic Surgery·D R MeldrumA H Harken
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·A S KristofS N Hussain
May 13, 1999·Biochimica Et Biophysica Acta·N Hogg, B Kalyanaraman
Jul 29, 1999·Chest·L D Hudson, K P Steinberg
Feb 26, 2000·Cardiovascular Research·P J Andrew, B Mayer
Apr 29, 2000·Intensive Care Medicine·C Brun-Buisson
May 12, 2000·Critical Care Medicine·A OberholzerL L Moldawer
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A TrifilieffC Bertrand
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S PervinG Chaudhuri
Aug 23, 2001·International Immunopharmacology·M J Thomassen, M S Kavuru
Oct 27, 2001·Molecular Aspects of Medicine·H J Forman, M Torres
Mar 2, 2002·The Lancet Infectious Diseases·T van der Poll
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiao-pei GaoAsrar B Malik
Apr 10, 2002·Critical Care Medicine·Habib M RazaviSanjay Mehta
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich A MausJürgen Lohmeyer
Jun 19, 2002·American Journal of Respiratory and Critical Care Medicine·Le Feng WangSanjay Mehta
Apr 3, 2003·The Journal of Clinical Investigation·Graciela AndoneguiPaul Kubes
Jul 25, 2003·The Journal of Thoracic and Cardiovascular Surgery·Babu V NaiduMichael S Mulligan
Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Daniel L Morgan, Cassandra J Shines
Nov 25, 2003·The American Journal of Pathology·Cecilia L SpeyerPeter A Ward
Apr 3, 2004·American Journal of Respiratory and Critical Care Medicine·Habib M RazaviSanjay Mehta
Jun 18, 2004·American Journal of Respiratory and Critical Care Medicine·Lorraine B WareMichael A Matthay
Jun 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rebecca M BaronMark A Perrella

❮ Previous
Next ❯

Citations

Apr 17, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·G CampanholleNiels Olsen Saraiva Camara
Jul 8, 2009·Critical Care : the Official Journal of the Critical Care Forum·Stefan LauerLars G Fischer
Dec 14, 2011·Free Radical Biology & Medicine·Peter R Kvietys, D Neil Granger
Apr 20, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pei-Yi ChuMing-Yie Liu
Apr 29, 2014·International Immunopharmacology·Gloria Gutiérrez-VenegasMaría Fernanda Ostoa-Pérez
Jun 22, 2016·Apoptosis : an International Journal on Programmed Cell Death·Zhi-Feng LiuLei Su
Nov 1, 2016·Cell and Tissue Research·Sean E GillRuud A W Veldhuizen
Dec 17, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·K S FarleyS Mehta
Mar 20, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Valerie ArpinoSean E Gill
Jul 20, 2016·International Journal of Molecular Medicine·Jae-Won LeeKyung-Seop Ahn
Apr 9, 2013·International Journal of Molecular Medicine·Peng GaoQingping Wen
Oct 19, 2013·Experimental and Therapeutic Medicine·Xiu-Juan LiuXiao-Chun Ma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved