Abstract
Multinucleated giant cells (MGC) are a prominent feature of some chronic inflammatory states in the lung. These cells are formed by macrophage fusion, but how this process relates to the kinetics of alveolar macrophage (AM) production and proliferation is not clear. In this serial study, we compare AM kinetics and MGC formation after instilling carbon, silica, asbestos, bleomycin, and saline into the lungs of mice. Animals were killed up to 16 weeks later with [3H]thymidine injected 1 h before death. Counts of AM and MGC were carried out after bronchoalveolar lavage, and cell labeling was assessed by autoradiography. All test substances induced an inflammatory response with equal AM numbers recovered up to 2 weeks. Subsequently, the number returned to normal after carbon but remained elevated in the other groups. After carbon the lung structure was normal, there was no increase in AM label, and no MGC formed. Bleomycin-injected lungs progressed to fibrosis with only a brief, small increase in AM labeling and no MGC formation. After silica, and particularly asbestos, the lungs showed fibrosis, and many granulomas with large MGC were seen. Lavaged AM from these lungs showed a significant increase in DNA synthesis after 2 weeks, f...Continue Reading
Citations
Oct 21, 2006·Journal of Leukocyte Biology·Andree SchmidtVeronika Grau
May 24, 2008·Anesthesiology·Maria AmigoniRoberto Latini
Jun 11, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Takenori IshidaAkio Kuroda
Apr 16, 1998·Environmental Health Perspectives·G J RosenthalP Simeonova
Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·S AndersonA P Mountford
Jun 18, 1999·Reviews on Environmental Health·G J RosenthalE Corsini
Dec 17, 2008·Clinical and Experimental Immunology·T C M Th van MaarsseveenP J van Diest
Dec 31, 1997·American Journal of Respiratory Cell and Molecular Biology·T SchneiderA C Issekutz
Jun 29, 2005·International Journal of Toxicology·P KumarathasanR Vincent
Sep 9, 2010·Cell and Tissue Research·David SchnebergerBaljit Singh
Sep 11, 2019·International Journal of Molecular Sciences·Fusheng JiangGuiyuan Lv
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thalie DevosseMarc Parmentier
Oct 3, 2017·Inhalation Toxicology·Breanne Y FarrisJenny R Roberts
May 25, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Sebastian J GraffiMichelle M Epstein
Aug 11, 2005·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Milan BenoSoterios Andreou Kyrtopoulos