Alveolar macrophage phagocytosis is impaired in children with poorly controlled asthma.

The Journal of Allergy and Clinical Immunology
Anne M FitzpatrickLou Ann S Brown

Abstract

Lower respiratory tract infection is a differentiating feature of children with poorly controlled asthma. Given the role of alveolar macrophages (AMs) in innate immunity, we hypothesized that AM phagocytosis might be impaired in poorly controlled asthma. Bronchoalveolar lavage fluid AMs were isolated from 28 asthmatic children (moderate asthma, n = 12; severe asthma, n = 16), 10 nonasthmatic children with chronic cough treated with inhaled corticosteroids, and 10 healthy adult control subjects. AMs were stimulated with LPS and exposed to fluorescein isothiocyanate-conjugated Staphylococcus aureus for 2 hours. Phagocytosis was quantified by using a phagocytic index (PI) calculated from the percentage of phagocytic cells multiplied by the relative fluorescence (RFU) units of S. aureus per cell. Apoptosis was determined from the percentage of cells positive for poly (adenosine diphosphate-ribose) polymerase. Phagocytosis as measured by using the unstimulated PI was decreased in subjects with poorly controlled asthma (healthy control subjects, 9330 +/- 3992 RFU; chronic cough, 9042 +/- 5976 RFU; moderate asthma, 4361 +/- 2536 RFU; severe asthma, 3153 +/- 1886 RFU; P < .001) and remained unchanged with LPS stimulation. Children with...Continue Reading

References

Sep 1, 1995·American Journal of Respiratory and Critical Care Medicine
Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·J L HankinsonK B Fedan
Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Sep 11, 2001·American Journal of Respiratory and Critical Care Medicine·S L JonesD R Taylor
Oct 31, 2002·Immunologic Research·Karen E Iles, Henry Jay Forman
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Dec 6, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xuejun J YinJoseph K H Ma
Feb 26, 2004·Journal of Medical Virology·Tuomas JarttiOlli Ruuskanen
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·Peter W HeymannRachel Chamberlain
Nov 9, 2004·Pediatric Research·Theresa W GauthierLou Ann S Brown
Jun 2, 2006·The European Respiratory Journal·S HodgeP N Reynolds
Dec 5, 2006·The Journal of Allergy and Clinical Immunology·Nino KhetsurianiW Gerald Teague
Dec 13, 2006·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN National Institutes of Health/National Heart, Lung, and Blood Institute Severe Asthma Research Program
Mar 14, 2007·The Journal of Allergy and Clinical Immunology·Merci M H KuselPeter D Sly

❮ Previous
Next ❯

Citations

May 8, 2010·Apoptosis : an International Journal on Programmed Cell Death·Olga KryskoClaus Bachert
Aug 23, 2012·Mucosal Immunology·J Balhara, A S Gounni
Dec 1, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher D LucasAdriano G Rossi
Jan 18, 2012·Current Opinion in Allergy and Clinical Immunology·Anne M FitzpatrickLeonard B Bacharier
Nov 3, 2011·Current Opinion in Pulmonary Medicine·Kian F Chung
Feb 7, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mark T KearnsR William Vandivier
Apr 13, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Theresa W GauthierLou Ann S Brown
Sep 16, 2011·Journal of Allergy·Aleksandra Szczawinska-Poplonyk
Aug 6, 2011·American Journal of Respiratory and Critical Care Medicine·Loren C DenlingerNizar N Jarjour
Nov 19, 2011·American Journal of Respiratory and Critical Care Medicine·Nizar N JarjourUNKNOWN NHLBI Severe Asthma Research Program (SARP)
Jul 28, 2012·American Journal of Respiratory and Critical Care Medicine·Olga SidelevaAnne E Dixon
Nov 13, 2012·American Journal of Respiratory and Critical Care Medicine·Loren C DenlingerUNKNOWN NHLBI Asthma Clinical Research Network Investigators
Apr 20, 2012·Respiratory Research·Qingling ZhangKian Fan Chung
Oct 27, 2010·Pediatric Research·Anne M FitzpatrickUNKNOWN NIH/NHLBI Severe Asthma Research Program
Sep 13, 2013·Nutrients·Hashim A PeriyalilLisa G Wood
Jun 29, 2014·Respiratory Research·Zhike LiangLouise E Donnelly
Mar 13, 2014·BMC Immunology·Carla WinklerJens M Hohlfeld
Feb 13, 2014·Toxins·Frederick T Guilford, Janette Hope
Dec 3, 2013·International Journal of Pharmaceutics·Yi-Bo WangRobert O Williams
Dec 1, 2013·Annals of the American Thoracic Society·Kian Fan Chung, Ian M Adcock
Apr 5, 2012·Chest·Louise E Donnelly, Peter J Barnes
Feb 5, 2013·The Journal of Allergy and Clinical Immunology·David M PyleMichelle A Gill
Sep 18, 2012·The Journal of Allergy and Clinical Immunology·Karl J StaplesRatko Djukanović
Aug 30, 2012·Clinics in Chest Medicine·Isabelle Tillie-LeblondJacques de Blic
Aug 1, 2012·The Journal of Allergy and Clinical Immunology·Kristina M JamesTina V Hartert
Jan 17, 2016·The Journal of Allergy and Clinical Immunology. in Practice·Anne M Fitzpatrick
Nov 1, 2013·Respiratory Medicine·D GibeonK F Chung
Apr 8, 2010·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN National Institutes of Health/National Heart, Lung, and Blood Institute's Severe Asthma Research Program
Feb 23, 2010·Journal of Microbiological Methods·Adam M PitzMartha J Gentry-Nielsen
Jan 10, 2009·The Journal of Allergy and Clinical Immunology·Stanley J Szefler
Mar 10, 2016·Seminars in Immunopathology·Aleksander M Grabiec, Tracy Hussell
Jan 3, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J L SimpsonUNKNOWN AMAZES Study Research Group
Jan 28, 2014·The Lancet. Respiratory Medicine·Kian Fan Chung
May 6, 2015·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Ina SintobinClaus Bachert
Aug 21, 2015·Current Allergy and Asthma Reports·Sumit GhoshJane M Schuh
Sep 14, 2015·Revista chilena de pediatría·Alberto Vidal G
Oct 21, 2014·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Jenna C PodjasekYoung J Juhn
Feb 26, 2014·Thorax·Rossa E BrughaJonathan Grigg

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

American Journal of Physiology. Lung Cellular and Molecular Physiology
Lou Ann S BrownTheresa W Gauthier
The Journal of Allergy and Clinical Immunology
Anne M FitzpatrickNational Institutes of Health/National Heart, Lung, and Blood Institute's Severe Asthma Research Program
© 2022 Meta ULC. All rights reserved