Alzheimer amyloid-beta peptides block the activation of C/EBPbeta and C/EBPdelta in glial cells

Biochemical and Biophysical Research Communications
Malin SamuelssonK Iverfeldt

Abstract

Members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors have been reported to be up-regulated in Alzheimer's disease. In the present study, we have investigated the effects of amyloid-beta (Abeta) peptides on C/EBPbeta and C/EBPdelta, previously shown to be induced by inflammatory stimuli in glial cells. Surprisingly, electrophoretic mobility shift assay showed that both Abeta(1-42) and Abeta(25-35) blocked C/EBP activation induced by the inflammatory cytokine interleukin-1beta (IL-1beta) or lipopolysaccharide (LPS) in mixed primary glial cell cultures from rat. Abeta also blocked IL-1beta- or LPS-induced C/EBP protein levels. The most prominent effects were observed on DNA binding activity and protein levels of C/EBPdelta. Our results demonstrate a dysregulation of C/EBP when glial cells are activated in the presence of Alzheimer Abeta peptides.

References

Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W S GriffinC Araoz
Oct 24, 1998·The Journal of Biological Chemistry·J Lekstrom-Himes, K G Xanthopoulos
Jun 12, 2002·The Journal of Biological Chemistry·Karie N DahlgrenMary Jo LaDu
Dec 13, 2003·The Journal of Biological Chemistry·Pelin Kelicen, Niclas Tindberg
Mar 9, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Diego J Garzon, Margaret Fahnestock

❮ Previous
Next ❯

Citations

Jun 29, 2011·Future Medicinal Chemistry·Vinicius Barreto da SilvaCarlos Henrique Tomich de Paula da Silva
Aug 13, 2013·The International Journal of Neuroscience·Zhiyou CaiLiang-Jun Yan
Mar 26, 2013·Neurobiology of Aging·Tony ValenteJosep Saura
Jan 1, 2021·Journal of Biomolecular Structure & Dynamics·Shibaji GhoshBishwajit Ganguly
Jun 19, 2021·Proceedings of the National Academy of Sciences of the United States of America·Zhourui WuKeqiang Ye
Oct 22, 2011·Neurobiology of Aging·Tony ValenteJosep Saura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Astrocytes & Amyloid

Astrocytes are thought to play a role in amyloid production and have been implicated in neurodegenerative diseases such as Alzheimer’s disease. Here is the latest research on the relationship between astrocytes and amyloid.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.