PMID: 6538056Jan 1, 1984Paper

Alzheimer paired helical filaments: bulk isolation, solubility, and protein composition

Acta Neuropathologica
K IqbalH M Wisniewski

Abstract

A method has been developed for the bulk isolation of Alzheimer neurofibrillary tangles (ANT) of paired helical filaments (PHF) from histopathologically confirmed cases of Alzheimer disease/senile dementia of the Alzheimer type (AD/SDAT). The fresh or frozen autopsied cerebral cortex affected with Alzheimer neurofibrillary changes is dissociated by homogenization and sieving through nylon bolting cloth and the ANT are separated by a combination of sucrose discontinuous density gradient centrifugation, glass bead column chromatography, and sodium dodecyl sulfate (SDS) treatment. The isolated ANT produce red-green birefringence when viewed through polarized light after staining with Congo red. Ultrastructurally, the isolated PHF are well preserved and have the dimensions of the PHF seen in situ. Two major Populations of ANT which exist in different proportions in AD/SDAT brains are identified on the basis of their solubility in SDS. The ANT I and the ANT II are soluble and insoluble respectively on treatment with 2% SDS at room temperature for 5 min. Solubilization of the ANT II requires several repeated extractions with a solution containing 10% each of SDS and beta-mercaptoethanol (BME) at 100 degrees C for 10 min. Sonication o...Continue Reading

References

Mar 1, 1979·Annals of Neurology·K WisniewskiH M Wisniewski
Dec 1, 1979·Annals of Neurology·I Grundke-IqbalK Iqbal
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G G GlennerJ D Termine
Sep 6, 1974·Brain Research·K IqbalR D Terry
Sep 1, 1970·Journal of the Neurological Sciences·B E TomlinsonM Roth
Jun 5, 1980·The New England Journal of Medicine·G G Glenner
Jan 1, 1984·Acta Neuropathologica·I Grundke-IqbalH M Wisniewski
Jan 1, 1981·Acta Neuropathologica·P A MerzK Iqbal

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Citations

Jan 1, 1994·Acta Neuropathologica·A RehmanI Grundke-Iqbal
Jan 1, 1991·Acta Neuropathologica·G P WangI Grundke-Iqbal
Jan 1, 1990·Acta Neuropathologica·I Grundke-IqbalJ G Joshi
Jan 1, 1984·Acta Neuropathologica·I Grundke-IqbalH M Wisniewski
Jan 1, 1984·Acta Neuropathologica·G P WangH M Wisniewski
Jan 1, 1985·Acta Neuropathologica·I Grundke-IqbalH M Wisniewski
Jan 1, 1985·Acta Neuropathologica·I Grundke-IqbalH M Wisniewski
Feb 1, 1992·Neurochemical Research·I L PayanW H Frey
Jan 1, 1987·Neurochemical Research·H M WisniewskiR Rubenstein
Feb 3, 1986·Klinische Wochenschrift·J UlrichJ Kahn
Mar 1, 1990·Environmental Geochemistry and Health·R C MoretzH M Wisniewski
Mar 1, 1990·Environmental Geochemistry and Health·H M WisniewskiJ W Shek
Apr 1, 1996·Molecular and Chemical Neuropathology·H G BernsteinA Rinne
Aug 1, 1994·Molecular Neurobiology·K IqbalI Grundke-Iqbal
Dec 6, 1991·Brain Research·G P WangI Grundke-Iqbal
Aug 15, 1988·FEBS Letters·E Montejo de GarciniJ Avila
Oct 1, 1992·Journal of the Neurological Sciences·N J CairnsP L Lantos
Sep 1, 1989·Neurobiology of Aging·A D Snow, T N Wight
Jan 1, 1994·Neurobiology of Aging·H Ksiezak-Reding, J S Wall
May 1, 1995·Neurobiology of Aging·R M BradyL I Binder
Sep 1, 1992·Neuroscience·P J GonzálezJ Avila
Jan 1, 1992·Brain Research Bulletin·G H FisherW H Frey
Jun 7, 2005·Mechanisms of Ageing and Development·Kate M WebberMark A Smith

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