AM-3K, an anti-macrophage antibody, recognizes CD163, a molecule associated with an anti-inflammatory macrophage phenotype

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Yoshihiro KomoharaMotohiro Takeya

Abstract

CD163 is a member of the scavenger receptor cysteine-rich superfamily restricted to the monocyte/macrophage lineage and is thought to be a useful marker for anti-inflammatory or alternatively activated macrophages. In this study we used mass spectrometric analysis to determine that the antigen recognized by the antibody AM-3K, which we previously generated as a tissue macrophage-specific monoclonal antibody, was CD163. An anti-inflammatory subtype of macrophages stimulated by dexamethasone or interleukin-10 showed strong reactivity for AM-3K and increased expression of CD163 mRNA. Immunohistochemical staining of routinely processed pathological specimens revealed that AM-3K recognized a specialized subpopulation of macrophages. In granulomatous diseases such as tuberculosis, sarcoidosis, or foreign body reactions, tissue macrophages around granulomas, but not component cells of the granulomas such as epithelioid cells and multinucleated giant cells, showed positive staining for AM-3K. In atherosclerotic lesions, scattered macrophages in diffuse intimal lesions were strongly positive for AM-3K, whereas foamy macrophages in atheromatous plaques demonstrated only weak staining. We therefore suggest that, in routine pathological sp...Continue Reading

References

Jan 1, 1988·International Archives of Allergy and Applied Immunology·R AndreesenG W Löhr
Aug 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L ZengK Takahashi
Oct 6, 1997·The European Respiratory Journal·E M MinshallQ Hamid
Nov 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V H RaoR H Finnell
Nov 30, 1999·Journal of Leukocyte Biology·M M Van den HeuvelR H Beelen
Feb 24, 2001·Nature·M KristiansenS K Moestrup
Oct 23, 2001·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·R Hernandez-PandoI Estrada-García
Feb 20, 2002·European Journal of Clinical Investigation·T RosklintL M Hultén
Dec 24, 2002·Trends in Immunology·Matyas SandorThomas A Wynn
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jan 30, 2003·Journal of Leukocyte Biology·David M Mosser
Mar 5, 2004·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G GamberiP Picci
Apr 13, 2004·Journal of Leukocyte Biology·Timothy H SulahianPaul M Guyre
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Mar 18, 2005·Annual Review of Immunology·P R TaylorS Gordon

❮ Previous
Next ❯

Citations

Jan 29, 2013·Acta Neuropathologica·Shailender SinghWolfgang Brück
Jan 9, 2009·Journal of Leukocyte Biology·Keun Seok SeoGregory A Bohach
Sep 11, 2009·Current Opinion in Lipidology·Jason L Johnson, Andrew C Newby
Feb 25, 2011·Science Translational Medicine·Jered B HaunRalph Weissleder
May 31, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jonas H GraversenSøren K Moestrup
Nov 30, 2013·Arthritis Research & Therapy·Kiran NistalaLucy R Wedderburn
Nov 23, 2011·Journal of Clinical and Experimental Hematopathology : JCEH·Yoshihiro KomoharaMotohiro Takeya
Sep 3, 2013·Journal of Clinical and Experimental Hematopathology : JCEH·Bing BaiYoshihiro Komohara
Dec 2, 2010·Rheumatology International·Wakana NakayamaHironobu Ihn
Nov 2, 2011·Chemical & Pharmaceutical Bulletin·Mona El-AasrToshihiro Nohara
Aug 24, 2012·Arthritis Research & Therapy·Lukasz StawskiMaria Trojanowska
Jun 26, 2015·Membranes·Jonas Heilskov Graversen, Søren Kragh Moestrup
Aug 19, 2014·International Journal of Cardiology·S PucciA Mauriello
Sep 29, 2015·Journal of Atherosclerosis and Thrombosis·Yoshihiro KomoharaMotohiro Takeya
Jul 9, 2011·Journal of Neuroimmunology·Zhiren ZhangHermann J Schluesener
Aug 25, 2009·Veterinary Immunology and Immunopathology·Andreas BeinekeWolfgang Baumgärtner
Jul 8, 2009·International Journal of Cancer. Journal International Du Cancer·Ivan ShaboJoar Svanvik
Mar 27, 2009·Experimental Dermatology·Nobuyo Higashi-KuwataHironobu Ihn
Jan 15, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Hirotake TakaiHiroaki Kataoka
Oct 11, 2007·Annals of Neurology·Assunta Dal BiancoHans Lassmann
May 29, 2008·International Journal of Cancer. Journal International Du Cancer·Ivan ShaboJoar Svanvik
Apr 9, 2009·Pathology International·Akira I HidaYoshito Sadahira
Feb 1, 2013·International Journal of Cancer. Journal International Du Cancer·Yu UsamiSatoru Toyosawa
Aug 14, 2012·American Journal of Reproductive Immunology : AJRI·Amy L JensenPatricia A Pioli
Jul 16, 2010·Journal of Cellular and Molecular Medicine·Heather M Wilson
Nov 3, 2011·European Journal of Haematology·Dok Hyun YoonJooryung Huh
Apr 2, 2011·Cancer Science·Yoshihiro KomoharaMotohiro Takeya

❮ Previous
Next ❯

Methods Mentioned

BETA
Electrophoresis
Enzyme-linked Immunosorbent Assay
immunoprecipitation
ELISA

Software Mentioned

Mascot

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.