AM5, a protein-associated polysaccharide, stimulates hematopoiesis and modulates the expression of endogenous hematopoietic growth factors in murine long-term bone marrow cultures

Stem Cells
G GüenecheaA Real

Abstract

We have investigated the mechanism behind the hematopoietic stimulation mediated by AM5, a protein-associated polysaccharide that stimulates in vivo the murine hematopoietic system. A dose-dependent increase in hematopoietic progenitors was observed in long-term bone marrow cultures (LTBMCs) treated in vitro with AM5. The stimulatory effect was more marked in colony-forming units-granulocyte-macrophage (CFU-GM) than in CFU-spleen (CFU-S) progenitors and also more significant in the supernatant than in the adherent layer. This stimulatory effect was reversible, and continuous stimulation with high doses of AM5 was conductive to a progressive exhaustion of the culture. The analysis of the CFU-GM stimulating activity present in the supernatant of AM5-treated cultures revealed a dose-dependent induction of granulocyte-macrophage colony-stimulating activity (GM-CSA), in contrast with control cultures in which no CSA was detected. Northern blots of LTBMC-adherent layers obtained after in vitro treatment with AM5 revealed a significant mRNA expression for interleukin 1 alpha (IL-1 alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage CSF (M-CSF) and granulocyte CSF (G-CSF), in contrast with adherent layers from...Continue Reading

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Citations

Jun 9, 1998·Stem Cells·D L GreinerL D Shultz
Nov 27, 2008·Journal of the Royal Society, Interface·Mark R PlaczekAthanasios Mantalaris
Mar 19, 2016·Intractable & Rare Diseases Research·Yang HanGuosheng Jiang
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Apr 9, 2013·Journal of Tissue Engineering and Regenerative Medicine·Pedro Z AndradeCláudia L da Silva
Jul 6, 2021·Frontiers in Immunology·Diego Fernández-LázaroMarcela González-Gross

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