Ambient GABA constrains the strength of GABAergic synapses at Cajal-Retzius cells in the developing visual cortex.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Knut Kirmse, Sergei Kirischuk

Abstract

At early stages of brain development, GABA plays a dual role. It fulfills important trophic functions and provides a major excitatory drive for the immature neuronal network. Here, we investigated whether GABA itself can limit the strength of excitatory GABAergic synapses on Cajal-Retzius (CR) cells in sagittal slices from the mouse visual cortex. (2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid (CGP55845), a specific GABAB receptor (GABABR) blocker, increased the frequency of spontaneous Ca2+ transients and spontaneous and miniature IPSCs (mIPSCs) but did not affect mIPSC amplitudes or kinetics. CGP55845 significantly increased evoked IPSC (eIPSC) amplitudes and decreased the paired-pulse ratio (PPR). Baclofen, a specific GABABR agonist, produced opposite effects. The size of the readily releasable pool was not affected by these GABABR modulators. The same CGP55845 actions were observed at physiological temperatures, but they were abolished after glutamate decarboxylase block with 3-mercaptopropionic acid (3-MP). These results indicate that presynaptic GABABRs dynamically regulate GABA release probability. SNAP-5114, a specific GABA transporter-2/3 (GAT-2/3) blocker, enhanced mIPSC ...Continue Reading

Citations

Jun 6, 2012·Pflügers Archiv : European journal of physiology·Anton DvorzhakSergei Kirischuk
Nov 15, 2006·Cerebral Cortex·Christian HennebergerRosemarie Grantyn
Feb 19, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anne E TakesianDan H Sanes
Apr 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zheng-Quan TangYong Lu
Mar 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pierre F Apostolides, Laurence O Trussell
Apr 6, 2012·Neurochemical Research·Alexei VerkhratskyVladimir Parpura
Sep 16, 2015·Journal of Medicinal Chemistry·Julianna KardosLászló Héja
Oct 4, 2008·The Journal of Physiology·Knut KirmseRosemarie Grantyn
May 29, 2012·Trends in Neurosciences·Sergei KirischukAlexei Verkhratsky
Jul 2, 2011·Pharmacology & Therapeutics·Jean-Luc GaiarsaChristophe Porcher
Apr 21, 2009·Current Opinion in Neurobiology·Ursula Koch, Anna K Magnusson
Dec 13, 2006·The European Journal of Neuroscience·Knut Kirmse, Sergei Kirischuk
Jan 18, 2014·Current Opinion in Neurobiology·Heiko J LuhmannWerner Kilb
Apr 28, 2010·The Journal of Physiology·Anton DvorzhakSergei Kirischuk
Aug 29, 2013·The Journal of Physiology·Sheela Toprani, Dominique M Durand
Oct 15, 2013·The European Journal of Neuroscience·Petr UnichenkoSergei Kirischuk
May 15, 2020·The European Journal of Neuroscience·Lisa FelixChristine R Rose
Mar 8, 2019·International Journal of Molecular Sciences·Charles NicaiseJacques Gilloteaux

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