Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells

Reproduction, Fertility, and Development
Christina R FerreiraRobert Graham Cooks

Abstract

Lipids play fundamental roles in mammalian embryo preimplantation development and cell fate. Triacylglycerol accumulates in oocytes and blastomeres as lipid droplets, phospholipids influence membrane functional properties, and essential fatty acid metabolism is important for maintaining the stemness of cells cultured in vitro. The growing impact that lipids have in the field of developmental biology makes analytical approaches to analyse structural information of great interest. This paper describes the concept and presents the results of lipid profiling by mass spectrometry (MS) of oocytes and preimplantation embryos, with special focus on ambient ionisation. Based on our previous experience with oocytes and embryos, we aim to convey that ambient MS is also valuable for stem cell differentiation analysis. Ambient ionisation MS allows the detection of a wide range of lipid classes (e.g. free fatty acids, cholesterol esters, phospholipids) in single oocytes, embryos and cell pellets, which are informative of in vitro culture impact, developmental and differentiation stages. Background on MS principles, the importance of underused MS scan modes for structural analysis of lipids, and statistical approaches used for data analysis a...Continue Reading

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Citations

Apr 23, 2015·Proceedings of the National Academy of Sciences of the United States of America·R Graham CooksValentina Pirro
Mar 17, 2017·Systems Biology in Reproductive Medicine·Alessandra A VirequeAna Carolina J Sá Rosa-E-Silva
Jul 23, 2016·International Journal of Molecular Sciences·Abdellah TebaniSoumeya Bekri
Mar 10, 2016·International Journal of Molecular Sciences·Nicola Pacini, Fabio Borziani
Oct 17, 2018·Journal of Mass Spectrometry : JMS·Camila Bruna de LimaR Graham Cooks
Oct 20, 2019·Journal of the American Society for Mass Spectrometry·Xu ZhaoXinrong Zhang
Jan 19, 2020·Analytical and Bioanalytical Chemistry·Fernanda Bertuccez CordeiroRobert Graham Cooks
Apr 11, 2020·Cytotechnology·Anna Bodzon-KulakowskaPiotr Suder
Jun 29, 2017·Rapid Communications in Mass Spectrometry : RCM·Fernanda B CordeiroR Graham Cooks

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Methods Mentioned

BETA
electron-capture dissociation
PCA

Software Mentioned

MS

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