Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry

Analytical Chemistry
Peter NemesAkos Vertes

Abstract

Mass spectrometry in conjunction with atmospheric pressure ionization methods enables the in vivo investigation of biochemical changes with high specificity and sensitivity. Laser ablation electrospray ionization (LAESI) is a recently introduced ambient ionization method suited for the analysis of biological samples with sufficient water content. With LAESI mass spectrometric analysis of chimeric Aphelandra squarrosa leaf tissue, we identify the metabolites characteristic for the green and yellow sectors of variegation. Significant parts of the related biosynthetic pathways (e.g., kaempferol biosynthesis) are ascertained from the detected metabolites and metabolomic databases. Scanning electron microscopy of the ablated areas indicates the feasibility of both two-dimensional imaging and depth profiling with a approximately 350 microm lateral and approximately 50 microm depth resolution. Molecular distributions of some endogenous metabolites show chemical contrast between the sectors of variegation and quantitative changes as the ablation reaches the epidermal and mesophyll layers. Our results demonstrate that LAESI mass spectrometry opens a new way for ambient molecular imaging and depth profiling of metabolites in biological t...Continue Reading

References

Mar 1, 1991·Rapid Communications in Mass Spectrometry : RCM·W EnsK G Standing
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·G Siuzdak
Oct 15, 1995·Analytical Chemistry·G A ValaskovicF W McLafferty
Feb 13, 2003·Chemical Reviews·Alfred Vogel, Vasan Venugopalan
Nov 22, 2005·Rapid Communications in Mass Spectrometry : RCM·Jentaie ShieaJan Sunner
Mar 18, 2006·Science·R Graham CooksJustin M Wiseman
Sep 15, 2006·Analytical Chemistry·Sheerin Khatib-ShahidiRichard M Caprioli
Oct 31, 2006·Plant Physiology·David M BraunR Frank Baker
Jan 16, 2007·Analytical Chemistry·Yue LiAkos Vertes
Mar 24, 2007·Analytical Chemistry·Peter NemesAkos Vertes
May 2, 2007·Mass Spectrometry Reviews·Liam A McDonnell, Ron M A Heeren
May 16, 2007·Analytical Chemistry·Klaus DreisewerdGottfried Pohlentz
May 24, 2007·Chemical Communications : Chem Comm·Dina R JustesR Graham Cooks
Jul 14, 2007·Journal of Mass Spectrometry : JMS·Mohammed A MeetaniFranco Basile
Jan 30, 2008·The Analyst·Yohannes H RezenomKermit K Murray
Mar 8, 2008·Nature Protocols·Justin M WisemanR Graham Cooks
Apr 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rohit ShroffJonathan Gershenzon

❮ Previous
Next ❯

Citations

Dec 29, 2010·Analytical and Bioanalytical Chemistry·Maria Careri, Alessandro Mangia
Jul 27, 2011·Analytical and Bioanalytical Chemistry·Allison L DillR Graham Cooks
Oct 12, 2012·Analytical and Bioanalytical Chemistry·Chhavi BhardwajLuke Hanley
Dec 5, 2012·Journal of the American Society for Mass Spectrometry·Guillaume RobichaudDavid C Muddiman
Feb 6, 2013·Journal of the American Society for Mass Spectrometry·Leonard NyadongAlan G Marshall
Apr 23, 2013·Journal of the American Society for Mass Spectrometry·Elaine C CabralDemian R Ifa
Sep 15, 2009·Journal of the American Society for Mass Spectrometry·Huanwen ChenRenato Zenobi
Apr 30, 2010·Journal of the American Society for Mass Spectrometry·Marion GirodR Graham Cooks
Apr 20, 2011·Analytical Chemistry·Yuqing LinAndrew G Ewing
Jan 11, 2013·Chemical Reviews·María Eugenia MongeFacundo M Fernández
Jan 15, 2013·The Analyst·David Gode, Dietrich A Volmer
Jan 13, 2010·Briefings in Functional Genomics·Adrian D Hegeman
Jul 20, 2010·Annual Review of Analytical Chemistry·Min-Zong HuangJentaie Shiea
Sep 14, 2010·Journal of Visualized Experiments : JoVE·Peter Nemes, Akos Vertes
Sep 14, 2010·Journal of Visualized Experiments : JoVE·Bindesh Shrestha, Akos Vertes
Nov 19, 2010·Bioanalysis·Richard J A Goodwin, Andrew R Pitt
Oct 30, 2010·Journal of Vacuum Science & Technology. A, Vacuum, Surfaces, and Films : an Official Journal of the American Vacuum Society·Slobodan MilasinovicLuke Hanley
Nov 19, 2010·Bioanalysis·Jun HanChristoph H Borchers
Jul 12, 2014·Annual Review of Analytical Chemistry·Paul Flanigan, Robert Levis
Apr 20, 2014·Journal of the American Society for Mass Spectrometry·Jeremy A BarryDavid C Muddiman
Feb 23, 2013·The Analyst·Rachel MasyukoPaul W Bohn
Jan 26, 2016·Methods : a Companion to Methods in Enzymology·Paul J Trim, Marten F Snel
Jun 29, 2010·Trends in Biotechnology·Ales Svatos
Feb 19, 2016·Analytical Chemistry·Erin GemperlineLingjun Li
Aug 18, 2009·Journal of Mass Spectrometry : JMS·Lee Chuin ChenKenzo Hiraoka
Sep 22, 2012·Mass Spectrometry Reviews·Chunping WuDemian R Ifa
Sep 29, 2012·Journal of Mass Spectrometry : JMS·Sung-Gun Park, Kermit K Murray
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Young Jin LeeBasil J Nikolau
Mar 19, 2011·Rapid Communications in Mass Spectrometry : RCM·Jiuming HeZeper Abliz
Nov 22, 2013·Journal of Mass Spectrometry : JMS·David CalligarisNathalie Y Agar
Sep 8, 2011·Mass Spectrometry Reviews·Zhong-Ping Yao
Nov 29, 2011·The Plant Journal : for Cell and Molecular Biology·Patrick J Horn, Kent D Chapman
Jul 4, 2013·Rapid Communications in Mass Spectrometry : RCM·Sung-Gun Park, Kermit K Murray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.