Ambient particulate matter enhances the pulmonary allergic immune response to house dust mite in a BALB/c mouse model by augmenting Th2- and Th17-immune responses

Physiological Reports
Alejandro R CastañedaKent E Pinkerton

Abstract

Ambient particulate matter (PM) exacerbates airway inflammation and hyper-reactivity in asthmatic patients. Studies show that PM has adjuvant-like properties that enhance the allergic inflammatory response; however, the mechanisms through which PM enhances these processes remain elusive. The objective of the study was to examine how ambient PM enhances the allergic immune response. Eight-week-old BALB/c mice were sensitized with house dust mite (HDM) or HDM and ambient particulate matter (PM, 2.5 μm; Sacramento, CA) to assess how PM modulates the development of adaptive immune responses against allergens. Both groups were challenged with HDM only. Bronchoalveolar lavage (BAL) was analyzed for extent of airway inflammation. Lung tissue was used for histological analysis, mucosubstance quantification, and heme oxygenase-1 (HO-1) localization/quantification. Gene expression was analyzed in whole lung to characterize immune markers of inflammation: cytokines, chemokines, antioxidant enzymes, and transcription factors. Cytokine and chemokine protein levels were quantified in whole lung to confirm gene expression patterns. Compared to HDM-only sensitization, exposure to PM during HDM sensitization led to significant immune cell recru...Continue Reading

References

Nov 1, 1980·British Journal of Industrial Medicine·M LippmannR E Albert
Oct 1, 1996·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·B Peterson, A Saxon
Sep 6, 2002·Chemico-biological Interactions·Michael S DenisonLaura Bonati
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Michael S Denison, Scott R Nagy
Feb 12, 2005·Journal of the Air & Waste Management Association·Jorn D HernerMichael J Kleeman
May 6, 2006·Environmental Health Perspectives·Rob McConnellJohn Peters
Sep 16, 2006·Biological Chemistry·Thomas Haarmann-Stemmann, Josef Abel
Feb 28, 2008·Nature Reviews. Immunology·Hannah J Gould, Brian J Sutton
Mar 26, 2008·Nature·Francisco J QuintanaHoward L Weiner
Dec 17, 2008·Current Opinion in Pulmonary Medicine·Christopher M EvansBurton F Dickey
Dec 17, 2008·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Kathleen MortimerIra Tager
Apr 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Patrick S WongFumio Matsumura
Jun 1, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F J Kelly, J C Fussell
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Sep 7, 2012·The Journal of Allergy and Clinical Immunology·Jennifer L Ingram, Monica Kraft
Sep 26, 2013·The Journal of Allergy and Clinical Immunology·Eric B BrandtGurjit K Khurana Hershey
Jul 23, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K M HewK C Nadeau
Oct 18, 2014·Frontiers in Immunology·Walker JulliardJoshua D Mezrich
Mar 10, 2015·The Journal of Allergy and Clinical Immunology·Eric B BrandtGurjit K Khurana Hershey
Jun 30, 2015·Allergo Journal International·Kilian Eyerich, Stefanie Eyerich
May 12, 2017·Journal of Toxicology and Environmental Health. Part a·Alejandro R CastañedaKent E Pinkerton

❮ Previous
Next ❯

Citations

Jun 17, 2020·Journal of Developmental Origins of Health and Disease·Delicia Shu-Qin OoiEvelyn Xiu-Ling Loo
Jul 28, 2020·Frontiers in Immunology·Anna I JoubertMartin Himly
Sep 19, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Hannah GiacominCarlos Atkins
Feb 20, 2020·International Journal of Environmental Research and Public Health·Jonathan K LondonKaren Andrade
Jun 23, 2020·Journal of Ophthalmology·Xiang-Tian MengHong-Yan Zhou
Nov 26, 2020·International Journal of Molecular Sciences·Odile Poulain-GodefroyPatricia de Nadai

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic absorption spectrophotometry
Assay
lavage
bronchoalveolar lavage
ELISA
PCR
protein assay

Software Mentioned

GraphPad PRISM
Primer3
ImageJ

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.