Ambient Profiling of Phenolic Content in Tea Infusions by Matrix-Assisted Ionization in Vacuum

Journal of the American Society for Mass Spectrometry
Robert B Cody

Abstract

Matrix-assisted ionization in vacuum (MAIV) was used to analyze the polyphenol content of ten different tea infusions. Nine different Camellia sinensis infusions were analyzed including three green teas, two black teas, two oolong teas, jasmine tea, and white tea. An infusion of rooibos (Aspalathus linearis) tea was also analyzed. Each freshly brewed tea was diluted 1:1 with methanol, and 100 ppm of phenolphthalein was added as an internal standard. An excess of 3-nitrobenzonitrile (NBN) was added to each vial, and the solution containing NBN crystals was analyzed by aspiration directly into the mass spectrometer sampling orifice. A working curve constructed for dilutions of catechin with phenolphthalein internal standard showed good linearity for five replicates of each concentration. The measured relative abundances of flavonoid polyphenols in each tea were in good agreement with previously reported values. Polyphenol content in tea infusions varied from 19.2 to 108.6 mg 100 mL-1. In addition to the expected catechin flavonoids, abundant quinic acid and gallic acid was detected in the C. sinensis infusions. Characteristic A. linearis flavonoids were detected in the rooibos tea. Graphical Abstract.

References

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Jan 1, 2016·Rapid Communications in Mass Spectrometry : RCM·Robert B Cody, A John Dane

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Citations

Jan 10, 2019·Rapid Communications in Mass Spectrometry : RCM·Chuping LeeChi-Kung Ni
May 14, 2020·Rapid Communications in Mass Spectrometry : RCM·Sarah TrimpinEllen D Inutan
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jeong-Ho OhSoon-Mi Shim
Dec 1, 2020·Journal of the American Society for Mass Spectrometry·Danielle R MannionMatthew S Wellons
Dec 4, 2020·Journal of the American Society for Mass Spectrometry·Khoa HoangMilan Pophristic

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