Ambient temperature enhanced freezing tolerance of Chrysanthemum dichrum CdICE1 Arabidopsis via miR398

BMC Biology
Yu ChenFadi Chen

Abstract

ICE (Inducer of CBF Expression) family genes play an important role in the regulation of cold tolerance pathways. In an earlier study, we isolated the gene CdICE1 from Chrysanthemum dichrum and demonstrated that freezing tolerance was enhanced by CdICE1 overexpression. Therefore, we sought to determine the mechanism by which ICE1 family genes participate in freezing tolerance. Using EMSA (Electrophoretic Mobility Shift Assay) and yeast one-hybrid assays, we confirmed that CdICE1 binds specifically to the MYC element in the CdDREBa promoter and activates transcription. In addition, overexpression of CdICE1 enhanced Arabidopsis freezing tolerance after transition from 23°C to 4°C or 16°C. We found that after acclimation to 4°C, CdICE1, like Arabidopsis AtICE1, promoted expression of CBFs (CRT/DRE Binding Factor) and their genes downstream involved in freezing tolerance, including COR15a (Cold-Regulated 15a), COR6.6, and RD29a (Responsive to Dessication 29a). Interestingly, we observed that CdICE1-overexpressing plants experienced significant reduction in miR398. In addition, its target genes CSD1 (Copper/zinc Superoxide Dismutase 1) and CSD2 showed inducible expression under acclimation at 16°C, indicating that the miR398-CSD pat...Continue Reading

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Citations

Jan 22, 2015·International Journal of Molecular Sciences·Chunyan GaoSumei Chen
Jul 18, 2015·Plant Physiology and Biochemistry : PPB·Peiling LiFadi Chen
Oct 22, 2016·The New Phytologist·Marinus Pilon
Jan 8, 2020·International Journal of Molecular Sciences·Xuan Lan Thi HoangNguyen Phuong Thao
Sep 15, 2020·Plant Signaling & Behavior·Rakesh Kumar VermaViswanathan Chinnusamy
Nov 15, 2019·Plant Physiology and Biochemistry : PPB·Zhaohe ZhangFadi Chen
Mar 17, 2021·Plant Cell Reports·Nguyen Nguyen ChuongNguyen Phuong Thao

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Methods Mentioned

BETA
ICEs
sumoylation
transgenic
reverse transcription PCR
electrophoretic mobility shift assay
one-hybrid
PCR
reverse
ICE
LightShift

Software Mentioned

PLACE
Bio
Rad iQ5 Optical System

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