Amebic cysteine proteinase 2 (EhCP2) plays either a minor or no role in tissue damage in acute experimental amebic liver abscess in hamsters

Parasitology Research
A Olivos-GarcíaR Pérez-Tamayo

Abstract

Amebic cysteine protease 2 (EhCP2) was purified from ethyl ether extracts of axenically grown trophozoites of Entamoeba histolytica strain HM1-IMSS. The purification procedure involved molecular filtration and electroelution. Sequence analysis of the purified product revealed EhCP2 and ubiquitin(s). Electrophoretic migration patterns, isoelectric point determination and Western blot studies failed to reveal other EhCP molecules. Polyclonal antibodies against the purified EhCP2 prepared in rabbits either stabilized or enhanced the enzyme activity in a dose-response manner. Purified EhCP2 was enclosed within inert resin microspheres (22-44 microm in diameter) and injected into the portal vein of normal hamsters. In the liver, the microspheres caused mild acute inflammation and occasional minimal necrosis of short duration. Sections of the liver were immunohistochemically stained with the anti-EhCP2 antibody and the microspheres were positive for only a very short period (1 h) after injection. Sections of experimental acute (1 day, 5 days) amebic liver abscess produced in hamsters were also stained with the anti-EhCP2 antibody; and amebas were intensely positive but no staining was observed at any time in the surrounding necrotic ...Continue Reading

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Citations

Jun 23, 2009·Medical Hypotheses·Rafael Campos-RodríguezJavier Ventura-Juárez
Apr 21, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Alfonso Olivos-GarcíaRuy Pérez-Tamayo
Jan 6, 2009·Annals of the New York Academy of Sciences·A GonzálezR Pérez-Tamayo
Dec 29, 2005·Archives of Medical Research·Ruy Pérez-TamayoCarlos Barba Ostria
Dec 29, 2005·Archives of Medical Research·Víctor Tsutsumi, Mineko Shibayama
May 13, 2005·Experimental Parasitology·María de Lourdes Caballero-GarcíaEnedina Jiménez-Cardoso

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