Amelioration of UVB-induced oxidative stress and inflammation in fat-1 transgenic mouse skin

Biochemical and Biophysical Research Communications
Hye-Won YumYoung-Joon Surh

Abstract

ω-3 polyunsaturated fatty acids (PUFAs), which are abundant in fish oils, are known to scavenge lipid peroxyl radicals and potentiate host immune defence. As UVB-induced oxidative stress and inflammation have been implicated in apoptotic cell death, this study was aimed to investigate the anti-inflammatory, anti-oxidative, and anti-apoptotic capacity of fat-1 transgenic mice capable of converting ω-6 to ω-3 PUFAs. Wild-type (WT) C57BL/6 mice and fat-1 mice were maintained on the AIN-93 diet supplemented with 10% safflower oil rich in ω-6 PUFAs for 5 weeks. The ω-3/ω-6 PUFA ratio was significantly higher in the dorsal skin of fat-1 mice than that in the WT mice. Upon single exposure to UVB (5.0 kJ/m2) radiation, fat-1 mice showed inflammatory as well as oxidative tissue damage and the expression of pro-inflammatory enzymes, cyclooxygenases-2 and inducible nitric oxide synthase in the skin to a much lesser extent than the WT mice. The protection of fat-1 mice from UVB-induced skin inflammation was associated with decreased phosphorylation of STAT3. Moreover, UVB-induced apoptosis was attenuated in fat-1 mouse skin. In comparison to WT animals, higher levels of Nrf2 and its target proteins, such as heme oxygenase-1, NAD(P)H:quinon...Continue Reading

References

Dec 1, 1994·Environmental Health Perspectives·P CeruttiP Amstad
Jan 1, 1995·Biochemical Society Symposium·R M Tyrrell
Apr 1, 1996·Current Opinion in Cell Biology·C L Carpenter, L C Cantley
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Virginie MaillardVéronique Chajès
Sep 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Katrin RamsauerPavel Kovarik
Nov 25, 2003·The Journal of Investigative Dermatology·Catherine S TrippAlice P Pentland
Feb 7, 2004·Nature·Jing X KangZhao B Kang
May 1, 2004·International Journal of Dermatology·Christina S SanderJens J Thiele
Sep 1, 2004·Free Radical Biology & Medicine·Dennis R Petersen, Jonathan A Doorn
Mar 8, 2005·Mutation Research·Farrukh AfaqHasan Mukhtar
Apr 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hon-Wai KoonCharalabos Pothoulakis
Jan 29, 2008·Photochemistry and Photobiology·Joydeb Kumar KunduYoung-Joon Surh
Apr 1, 2010·Pharmaceutical Research·Joydeb Kumar Kundu, Young-Joon Surh
Mar 7, 2012·Free Radical Biology & Medicine·Joydeb Kumar Kundu, Young-Joon Surh
Jan 1, 2011·Biochemia Medica·Annalisa Del PreteAlberto Mantovani

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