Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Xuanyu LuXianghong Luan

Abstract

Matrix molecules such as the enamel-related calcium-binding phosphoprotein ameloblastin (AMBN) are expressed in multiple tissues, including teeth, bones, and cartilage. Here we have asked whether AMBN is of functional importance for timely long bone development and, if so, how it exerts its function related to osteogenesis. Adolescent AMBN-deficient mice (AMBN(Δ5-6) ) suffered from a 33% to 38% reduction in femur length and an 8.4% shorter trunk spinal column when compared with WT controls, whereas there was no difference between adult animals. On a cellular level, AMBN truncation resulted in a shortened growth plate and a 41% to 49% reduction in the number of proliferating tibia chondrocytes and osteoblasts. Bone marrow stromal cells (BMSCs) isolated from AMBN mutant mice displayed defects in proliferation and differentiation potential as well as cytoskeleton organization. Osteogenesis-related growth factors, such as insulin-like growth factor 1 (IGF1) and BMP7, were also significantly (46% to 73%) reduced in AMBN-deficient BMSCs. Addition of exogenous AMBN restored cytoskeleton structures in AMBN mutant BMSCs and resulted in a dramatic 400% to 600% increase in BMP2, BMP7, and Col1A expression. Block of RhoA diminished the eff...Continue Reading

References

Aug 15, 1994·Microscopy Research and Technique·E B Hunziker
Feb 23, 1996·The Journal of Biological Chemistry·P H KrebsbachY Yamada
Jun 22, 2000·Journal of Cellular Biochemistry·R T FranceschiR B Rutherford
Dec 12, 2001·Endocrine Reviews·J NakaeD Accili
Sep 10, 2002·Developmental Biology·Udayan GuhaJohn A Kessler
Sep 26, 2002·Annual Review of Anthropology·B Bogin
Aug 2, 2003·Molecular and Cellular Endocrinology·Liqiang TouJoseph M Alexander
Dec 4, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Margarita Zeichner-DavidPablo Bringas
Aug 19, 2004·Molecular Ecology·David L EricksonDonald Price
Dec 8, 2004·The Journal of Cell Biology·Satoshi FukumotoYoshihiko Yamada
Apr 14, 2006·Cells, Tissues, Organs·Satoshi FukumotoYoshihiko Yamada
May 6, 2006·European Journal of Oral Sciences·Maggie Zeichner-DavidPablo Bringas
Dec 23, 2006·European Journal of Oral Sciences·Axel SpahrGita Pezeshki
Apr 10, 2009·Current Pharmaceutical Design·Chiara Gentili, Ranieri Cancedda
Apr 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Rima M WazenAntonio Nanci
Jun 25, 2009·Pharmacological Reviews·Hannu JärveläinenRisto Penttinen
Aug 4, 2009·Cell and Tissue Research·Toshihisa Komori
Jan 2, 2010·Experimental Cell Research·Michael BeyelerBeat Trueb
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
Jun 25, 2010·Journal of Periodontal Research·J NuñezH Arzate
Jul 27, 2011·Current Molecular Pharmacology·Daniel D Bikle, Yongmei Wang
Jan 25, 2012·European Journal of Oral Sciences·Xuanyu LuThomas G H Diekwisch
Feb 22, 2012·European Journal of Oral Sciences·Tomáš WaldJiří Vondrášek
Jan 25, 2012·European Journal of Oral Sciences·Youbin ZhangXianghong Luan
Jun 16, 2012·The Journal of Endocrinology·Katherine A StainesColin Farquharson
Jan 4, 2013·Cold Spring Harbor Perspectives in Biology·Fanxin Long, David M Ornitz
Feb 6, 2013·Frontiers in Endocrinology·Candice G T TahimicDaniel D Bikle
Apr 30, 2013·Journal of Dental Research·P AtsawasuwanT G H Diekwisch
Mar 8, 2014·Small GTPases·Eeva Kaisa Rajakylä, Maria K Vartiainen
May 27, 2014·Human Molecular Genetics·James A PoulterAlan J Mighell

❮ Previous
Next ❯

Citations

Feb 24, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Xuanyu LuXianghong Luan
Mar 16, 2017·Journal of Cellular Biochemistry·Mai OtsukaTatsuji Nishihara
Aug 12, 2017·Frontiers in Physiology·Øystein StakkestadJanne E Reseland
Aug 9, 2020·Journal of Dental Research·M FrancisX Luan
Feb 23, 2017·Frontiers in Physiology·Øystein StakkestadJanne Elin Reseland
Aug 29, 2020·Bone·Paul H SchlesingerHarry C Blair
Jul 31, 2021·Journal of Dental Research·W MaT G H Diekwisch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.