American oyster, Crassostrea virginica, expresses a potent antibacterial histone H2B protein.

Marine Biotechnology
Jung-Kil SeoE J Noga

Abstract

An antibacterial protein was purified from acidified gill extract of a bivalve mollusk, the American oyster (Crassostrea virginica). Protein isolation was best accomplished by briefly boiling the tissues in a weak acetic acid solution. Adding protease inhibitors while boiling did not have a major effect on activity recovery. In contrast, use of only protease inhibitors (without boiling) resulted in virtually no recovery of this activity. The amino acid sequence of this antibacterial protein was identified as a histone H2B and was designated cvH2B. cvH2B had potent activity against gram-negative bacteria, including the human pathogens Vibrio parahaemolyticus and Vibrio vulnificus, which commonly reside in oyster tissues. We estimated that the concentration of this protein was well within the concentration that was inhibitory to these bacterial pathogens in vitro. This is the first report of the antimicrobial function of histone H2B from any mollusk.

References

Jul 1, 1993·Infection and Immunity·P S HiemstraR I Lehrer
Jul 2, 1999·Applied and Environmental Microbiology·F J GenthnerW S Fisher
Jun 9, 2001·Biochemical and Biophysical Research Communications·R C RichardsK V Ewart
Feb 22, 2002·European Journal of Biochemistry·Yuqin WangJan Johansson
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hun Sik KimSun Chang Kim
Aug 8, 2002·The Biochemical Journal·Jorge M O FernandesValerie J Smith
Dec 4, 2003·Biochemical and Biophysical Research Communications·Hiroaki KawasakiJ Michael Conlon
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qing-Gang XueJerome F La Peyre
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·J Andy Tincu, Steven W Taylor
Dec 21, 2004·European Journal of Biochemistry·Séverine A PatatKevin L Schey
Jan 11, 2005·Current Protein & Peptide Science·Dawn M E BowdishRobert E W Hancock
Nov 22, 2005·Biochemical and Biophysical Research Communications·Jung-Kil SeoEdward J Noga
Jun 1, 1993·Applied and Environmental Microbiology·J A OlafsenG Høvik Hansen
Jan 4, 2006·Biochemical and Biophysical Research Communications·Umaporn SilphaduangYoshinori Mine
Apr 21, 2006·Physics in Medicine and Biology·Oliver Jäkel
May 25, 2006·Proceedings. Biological Sciences·Kerry M OliverMartha S Hunter
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Missag H Parseghian, Keith A Luhrs
Jan 16, 2007·Developmental and Comparative Immunology·Dusan PalićJames A Roth
Apr 10, 2007·Food Microbiology·Yi-Cheng Su, Chengchu Liu
Mar 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Edward J NogaShannon Kozlowicz

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Citations

Jan 3, 2013·Microbial Ecology·Brett Froelich, James D Oliver

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