Amide hydrogen exchange shows that malate dehydrogenase is a folded monomer at pH 5

Protein Science : a Publication of the Protein Society
J Chen, D L Smith

Abstract

Although there is general agreement that native mitochondrial malate dehydrogenase (MDH) exists as a dimer at pH 7, its aggregation state at pH 5 is less certain. The present amide hydrogen exchange study was performed to determine whether MDH remains a dimer at pH 5. To detect pH-induced changes in solvent accessibility, MDH was exposed to D(2)O at pH 5 or 7, then fragmented with pepsin into peptides that were analyzed by mass spectrometry. Even after adjustments for the effect of pH on the intrinsic rate of hydrogen exchange, large increases in deuterium levels were found at pH 5 only in peptic fragments derived from the subunit binding surface of MDH. In parallel experiments, elevated deuterium levels were also found in the same regions of MDH monomer trapped inside a mutant form of the chaperonin GROEL: This selective increase in hydrogen exchange rates, which was attributed to increased solvent accessibility of these regions, provides new evidence that MDH is a monomer at pH 5.

References

Jun 14, 1978·Biochemical and Biophysical Research Communications·D C WoodJ H Harrison
Feb 24, 1976·Biochemistry·J D Shore, S K Chakrabarti
Jun 1, 1991·Archives of Biochemistry and Biophysics·J S Steffan, L McAlister-Henn
May 15, 1985·Analytical Biochemistry·J J EnglanderS W Englander
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Apr 30, 1966·Nature·T DévényiR G Wolfe
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Oct 29, 1982·Molecular and Cellular Biochemistry·C WoodwardE Tüchsen
Sep 1, 1993·Proteins·Y BaiS W Englander
Apr 1, 1993·Protein Science : a Publication of the Protein Society·Z Zhang, D L Smith
Feb 1, 1997·Journal of Mass Spectrometry : JMS·D L SmithZ Zhang
Oct 29, 1998·Protein Science : a Publication of the Protein Society·S A SánchezD M Jameson
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J G MandellE A Komives
Aug 19, 1999·Protein Science : a Publication of the Protein Society·R Li, C Woodward
Apr 7, 2000·Advances in Protein Chemistry·A K Chamberlain, S Marqusee
Jan 1, 1994·Journal of the American Society for Mass Spectrometry·Y Liu, D L Smith

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Citations

Nov 19, 2003·Journal of Molecular Biology·John K Chik, David C Schriemer
Jul 14, 2009·Biochemistry·T Michael Sabo, Muriel C Maurer
Jan 9, 2010·Biochimica Et Biophysica Acta·Izabela Rutkowska-WlodarczykJakub Wlodarczyk
Aug 21, 2014·PloS One·Himadri Biswas, Rajagopal Chattopadhyaya

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