PMID: 7543548Aug 1, 1995Paper

Amiloride blocks a keratinocyte nonspecific cation channel and inhibits Ca(++)-induced keratinocyte differentiation

The Journal of Investigative Dermatology
T MauroP A Pappone

Abstract

Proliferation and differentiation in many cells are linked to specific changes in transmembrane ion fluxes. Previously, we have identified a nonspecific cation channel in keratinocytes, which is permeable to and activated by Ca++. To test whether this cation channel might serve as a pathway for Ca++ entry, we examined the effect of blocking this channel on membrane currents, markers of differentiation, and intracellular Ca++. In patch clamp studies, 10(-8) to 10(-6) M amiloride decreased the single-channel open probability. The same concentrations of amiloride inhibited the calcium-induced formation of cornified envelopes and activity of transglutaminase in a dose-dependent fashion. Amiloride inhibited the long-term rise of intracellular Ca++ induced by raised extracellular Ca++, without blocking the initial increase of intracellular Ca++. Amiloride at concentrations of 10(-7) to 10(-3) M did not change the resting intracellular pH of keratinocytes, although concentrations of 10(-6) M or greater inhibited the recovery from NH4(+)-induced acidification. To test whether the effect of amiloride was toxic, we measured DNA synthesis in the presence or absence of amiloride. DNA synthesis was unchanged, suggesting that amiloride's act...Continue Reading

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Citations

Sep 5, 2002·Journal of Cellular Physiology·Donna R TrollingerRichard Nuccitelli
Aug 23, 2002·Experimental Dermatology·V H Nguyen, F Markwardt
Mar 7, 2003·American Journal of Physiology. Cell Physiology·Ralf BurgstahlerChristian Alzheimer
May 18, 2005·Skin Pharmacology and Physiology·E Ivanchenko, F Markwardt
Jul 4, 2003·The Journal of Biological Chemistry·Patricia A LaddDiane S Keeney
Nov 28, 2007·The Journal of Biological Chemistry·Roch-Philippe CharlesEdith Hummler
Jan 6, 1999·Experimental Neurology·H Y ChiangS T Hsieh
Sep 21, 2000·The Journal of Investigative Dermatology·A DascaluR Korenstein
Nov 26, 1999·The Journal of Investigative Dermatology·Y OdaT Mauro
Mar 29, 2002·The Journal of Investigative Dermatology·T MauroE Hummler
Jul 4, 2003·The Journal of Investigative Dermatology·Sahba FatheraziKenneth T Izutsu
Jun 1, 1997·The Journal of Investigative Dermatology·T MauroP A Pappone
Apr 21, 2005·Journal of Cell Science·Christine E Pullar, R Rivkah Isseroff

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