Amine functionalization of cholecyst-derived extracellular matrix with generation 1 PAMAM dendrimer

Biomacromolecules
Jeffrey C Y ChanAbhay S Pandit

Abstract

A method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy ana...Continue Reading

References

Mar 1, 1994·Journal of Biomedical Materials Research·P B van WachemP Nieuwenhuis
Jun 1, 1993·Analytical Biochemistry·E Curotto, F Aros
Apr 1, 1996·Biomaterials·L H Olde DaminkJ Feijen
May 29, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M J WissinkJ Feijen
Jul 11, 2000·Pharmaceutical Research·J E BabenseeA G Mikos
Mar 23, 2001·American Journal of Obstetrics and Gynecology·M MeinertA Malmström
Apr 20, 2001·Journal of Biomedical Materials Research·K BilliarN Bachrach
Jun 21, 2001·Endothelium : Journal of Endothelial Cell Research·J P HoddeS F Badylak
Apr 2, 2002·The Journal of Surgical Research·Stephen BadylakRobert Morff
Sep 27, 2002·Seminars in Cell & Developmental Biology·Stephen F Badylak
Feb 18, 2003·Molecular Biology of the Cell·Frederick GrinnellGabriella Skuta
Jul 31, 2003·Journal of Biomedical Materials Research. Part a·J M UnsworthK M Shakesheff
Sep 2, 2003·Pharmaceutical Research·Ruth R Chen, David J Mooney
Oct 7, 2003·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Zuwei MaJiacong Shen
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Cahir A McDevittRochelle M Cutrone
Apr 6, 2004·Carbohydrate Research·Willem F WolkersJohn H Crowe
Apr 28, 2004·Bioelectrochemistry·D M DomańskiM Bryszewska
Apr 1, 1997·Journal of Materials Science. Materials in Medicine·C S OsborneM H Grant
Aug 16, 2005·International Journal of Pharmaceutics·Aristarchos PapagiannarosCostas Demetzos
Aug 19, 2005·Journal of Biomedical Materials Research. Part a·X Duan, H Sheardown
Nov 26, 2005·The Anatomical Record. Part B, the New Anatomist·Stephen F Badylak
Dec 20, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L ButtafocoJ Feijen
May 3, 2006·The Journal of Surgical Research·Rainier KoGary C Lantz
Dec 26, 2006·International Journal of Pharmaceutics·Mohammad NajlahAntony D'Emanuele
Feb 3, 2007·Journal of Biomedical Materials Research. Part a·James C CoburnAbhay Pandit
Feb 27, 2007·Journal of Biomedical Materials Research. Part a·Jerome A HenryPeter Neuenschwander
May 15, 2007·Advanced Drug Delivery Reviews·Soo-Hong Lee, Heungsoo Shin

❮ Previous
Next ❯

Citations

Feb 27, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·J A Henry, A Pandit
Feb 1, 2011·Biomaterials·Estelle C CollinAbhay S Pandit
Sep 30, 2010·Chemical Society Reviews·Meredith A Mintzer, Mark W Grinstaff
Feb 21, 2014·Journal of Tissue Engineering·Deepa ReviThapasimuthu Vijayamma Anilkumar
Jan 18, 2018·Toxicologic Pathology·Dhanush Krishna Balakrishnan-NairThapasimuthu Vijayamma Anilkumar
Sep 30, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yang LeiYunbing Wang
Feb 16, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Kalpana NagpalPradeep Kumar
Mar 25, 2014·Expert Review of Medical Devices·Luis M DelgadoDimitrios I Zeugolis
Oct 9, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valentina BeghettoAlberto Scrivanti
Apr 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Abhishek MandalThotapalli P Sastry
Sep 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·William H C TiongAbhay Pandit

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.