PMID: 5912214Jun 1, 1966

Amino acid accumulation and incorporation in rat intestine in vitro

The Journal of Physiology
J R Bronk, D S Parsons

Abstract

1. Rings of rat jejunum incubated in vitro accumulate a mixture of amino acids at a rate of about 3 mumoles/cm. hr. The rate of incorporation of the accumulated amino acid into the tissue protein corresponds to a rate of synthesis of 50% of the protein of the whole wall in five days.2. Replacement of the Na(+) in the NaCl of the incubation medium by choline or by Li(+) did not prevent amino acid accumulation by the tissue. However, replacement of the Na(+) by K(+) prevented the accumulation.3. The accumulation of amino acids by rat jejunum in vitro proceeded at normal rates not only in the presence in the incubation medium of oxygen tensions below 10 Torr but also in the presence of 2,4-dinitrophenol. Reasons are given for supposing that the findings are compatible with the view that the energy upon which depend the processes of amino acid accumulation by the tissue could be derived from the movement of ions across cellular boundaries.4. The amino acid incorporation into the tissue proteins was reduced to one tenth of the control rate in the presence of 2,4-dinitrophenol or by hypoxia so that the processes of incorporation depend upon energy derived from oxidative metabolism. In the presence of oligomycin the tissue respiration...Continue Reading

Citations

Jul 1, 1997·The Proceedings of the Nutrition Society·J van der Meulen, A J Jansman
Feb 1, 1969·Journal of Cellular Physiology·R W Merriam, G Pollack
Apr 15, 1969·Klinische Wochenschrift·D M Matthews

Related Concepts

Carbon Isotopes
Choline Hydroxide
Dinitrophenols
Glucose, (beta-D)-Isomer
Intestinal Absorption
Jejunum
Leucine
Lithium
Lysine Hydrochloride
Oligomycins

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.