Amino Acid Biosignature in Plasma among Ischemic Stroke Subtypes

BioMed Research International
Vânia A M GoulartRodrigo R Resende

Abstract

Ischemic stroke is a neurovascular disorder caused by reduced or blockage of blood flow to the brain, which may permanently affect motor and cognitive abilities. The diagnostic of stroke is performed using imaging technologies, clinical evaluation, and neuropsychological protocols, but no blood test is available yet. In this work, we analyzed amino acid concentrations in blood plasma from poststroke patients in order to identify differences that could characterize the stroke etiology. Plasma concentrations of sixteen amino acids from patients with chronic ischemic stroke (n = 73) and the control group (n = 16) were determined using gas chromatography coupled to mass spectrometry (GC-MS). The concentration data was processed by Partial Least Squares-Discriminant Analysis (PLS-DA) to classify patients with stroke and control. The amino acid analysis generated a first model able to discriminate ischemic stroke patients from control group. Proline was the most important amino acid for classification of the stroke samples in PLS-DA, followed by lysine, phenylalanine, leucine, and glycine, and while higher levels of methionine and alanine were mostly related to the control samples. The second model was able to discriminate the stroke...Continue Reading

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Citations

Nov 23, 2019·Metabolomics : Official Journal of the Metabolomic Society·Chaofu KeYonghong Zhang
Oct 15, 2019·Klinicheskaia laboratornaia diagnostika·A A ZhlobaYu S Polushin
Apr 30, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yunjie WangTao Pang

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