PMID: 1212197Sep 1, 1975Paper

Amino acid biosynthesis in mixed rumen cultures

The Biochemical Journal
F D SauerS Mahadevan

Abstract

Mixed rumen micro-organisms, maintained in continuous culture readily incorporated labelled HCO3- and acetate into amino acids. Labelled propionate, in contrast, was utilized only for isoleucine biosynthesis, but failed to label other amino acids to any significant extent. Evidence was obtained showing that in these mixed, i.e. symbiotic, cultures foward tricarboxylic acid-cycle reactions only proceed to 2-oxoglutarate. 14C distribution in amino acids clearly shows that 2-oxoglutarate is not oxidized further by tricarboxylic acid-cycle enzymes. Instead, acetate is carboxylated to pyruvate which is then carboxylated to oxaloacetate. Oxaloacetate equilibrates with fumarate and thereby carbon atoms 1 and 4 as well as carbon atoms 2 and 3 are randomized. Evidence was also obtained for the carboxylation of propionate to 2-oxobutyrate, isovalerate to 4-methyl-2-oxopentanoate, phenylacetate and hydroxyphentlacetate to the corresponding phenyl- and hydroxyphenyl-pyruvic acids and succinate to 2-oxoglutarate. Of the amino acid precursors investigated, only 3-hydroxypyruvate, the precursor of serine, appeared to be synthesized via an oxidative step, i.e. 3-phosphoglyceric acid to 3-phosphohydroxypyruvic acid. Most 2-oxo precursors of ami...Continue Reading

Citations

Apr 1, 1982·Critical Reviews in Microbiology·P N Hobson, R J Wallace
Jan 1, 1996·Critical Reviews in Microbiology·S C RickeD J Nisbet
May 1, 1982·Critical Reviews in Microbiology·P N Hobson, R J Wallace
Jun 1, 1995·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A D KeefeS L Miller
Feb 1, 1980·The Proceedings of the Nutrition Society·D I Demeyer, C J Van Nevel
Aug 21, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R H DunstanW Greenaway
Mar 1, 1979·The British Journal of Nutrition·J B RoweR A Leng
Sep 10, 2019·Journal of Animal Science·John L ViciniKatherine A Karberg
Aug 25, 2020·ELife·Charles Ar CottonArren Bar-Even
Mar 7, 2021·Nutrients·Matthew D WatsonGregory J Grosicki
Jun 27, 2000·The Journal of Nutrition·C C Metges
May 13, 1999·Journal of Bacteriology·M HochuliT Szyperski
Jun 1, 1986·Applied and Environmental Microbiology·R Onodera
Sep 1, 1979·Microbiological Reviews·D C Yoch, R P Carithers
Jun 19, 2021·Scientific Reports·Jessica Moraes MalheirosLuciana Correia de Almeida Regitano
Apr 14, 2021·Annual Review of Plant Biology·Hiroshi A Maeda, Alisdair R Fernie
Jul 12, 1976·Biochemical and Biophysical Research Communications·R S Bush, F D Sauer

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