Amino acid contents along the visual and equatorial axes of a pig lens by Raman spectroscopy

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
C Medina-GutiérrezR Sato-Berrú

Abstract

Using near infrared Raman microspectroscopy with laser light of 830 nm, the distribution of amino acids along the visual and equatorial axes of a normal pig lens was studied. The classification of pig lens Raman spectra in these axes was performed using principal component analysis and linear discriminant analysis. The analysis of the scattered light selectively collected from point to point, along the visual axis, indicated that the tyrosine and tryptophan increases and then, at approximately 4 mm position, decreases. Moreover, in the equatorial plane, the nuclear part has the highest concentration of these amino acids. However, the phenylalanine content increases from anterior to posterior cortex of the lens as long as in the equatorial axis it slightly increases and then at approximately 2-2.3 mm position, decreases. The changes in amino acid conformation along the visual axis, similarly to the changes in protein conformation, may explain the refractive gradient of the lens.

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May 19, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·C Medina-GutiérrezO Barbosa-García

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Citations

Nov 22, 2008·Journal of Biomedical Optics·Nagapratima KunapareddyJudith R Mourant
Feb 25, 2009·Annals of the New York Academy of Sciences·Istvan BercziKalman Kovacs

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