Amino acid-dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen-limited conditions and identification of AspB (Cj0762), essential for growth on glutamate.

Molecular Microbiology
Edward GuccioneDavid J Kelly

Abstract

Amino acids are key carbon and energy sources for the asaccharolytic food-borne human pathogen Campylobacter jejuni. During microaerobic growth in amino acid rich complex media, aspartate, glutamate, proline and serine are the only amino acids significantly utilized by strain NCTC 11168. The catabolism of aspartate and glutamate was investigated. An aspartase (aspA) mutant (unable to utilize any amino acid except serine) and a Cj0762c (aspB) mutant lacking aspartate:glutamate aminotransferase (unable to utilize glutamate), were severely growth impaired in complex media, and an aspA sdaA mutant (also lacking serine dehydratase) failed to grow in complex media unless supplemented with pyruvate and fumarate. Aspartase was shown by activity and proteomic analyses to be upregulated by oxygen limitation, and aspartate enhanced oxygen-limited growth of C. jejuni in an aspA-dependent manner. Stoichiometric aspartate uptake and succinate excretion involving the redundant DcuA and DcuB transporters indicated that in addition to a catabolic role, AspA can provide fumarate for respiration. Significantly, an aspA mutant of C. jejuni 81-176 was impaired in its ability to persist in the intestines of outbred chickens relative to the parent st...Continue Reading

References

Oct 1, 1986·Applied and Environmental Microbiology·H N WestfallE Weiss
Apr 1, 1986·Journal of Clinical Microbiology·M A KarmaliP C Fleming
May 1, 1984·The Journal of Urology·J S WheelerR J Krane
Jun 1, 1993·European Journal of Biochemistry·P K MehtaP Christen
Jan 1, 1997·Microbiology·Julian M Ketley
Mar 15, 1997·Biochimica Et Biophysica Acta·G L MendzD J Meek
Oct 10, 1998·Journal of Bacteriology·A H van VlietJ M Ketley
Nov 5, 1999·Clinics in Laboratory Medicine·P I Fields, D L Swerdlow
Jan 5, 2000·Microbes and Infection·T M Wassenaar, M J Blaser
Aug 9, 2002·The Journal of Biological Chemistry·Ingo G JanauschGottfried Unden
Mar 12, 2005·Biochimie·S AugerI Martin-Verstraete
Jul 8, 2005·Applied and Environmental Microbiology·A V Karlyshev, B W Wren
Jul 25, 2006·Infection and Immunity·Dirk HofreuterJorge E Galán
Sep 18, 2007·Journal of Bacteriology·Bruce M PearsonArnoud H M van Vliet
Apr 22, 2008·Applied and Environmental Microbiology·Chris CowardDuncan J Maskell

❮ Previous
Next ❯

Citations

Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Martin StahlAlain Stintzi
Nov 10, 2013·Genome Biology and Evolution·Zuowei WuQijing Zhang
Jun 23, 2009·Applied and Environmental Microbiology·Christina S VeggeHanne Ingmer
Jun 5, 2012·Applied and Environmental Microbiology·Caroline P A de HaanMarja-Liisa Hänninen
Apr 7, 2010·Infection and Immunity·Damien LeducHilde de Reuse
Oct 3, 2009·Infection and Immunity·Veronica NovikJorge E Galán
Jan 5, 2011·Journal of Bacteriology·Wayne T Muraoka, Qijing Zhang
Jul 1, 2011·Veterinary Research·David HermansFrank Pasmans
Nov 3, 2011·BMC Genomics·Aline MetrisArnoud H M van Vliet
Sep 24, 2009·Antonie van Leeuwenhoek·D Nathan JacksonJ T Trevors
Sep 10, 2013·Applied and Environmental Microbiology·Virginie DufourChristine Baysse
Sep 17, 2013·Journal of Cellular Biochemistry·Jamie A Meadows, Matthew J Wargo
Apr 29, 2009·Microbial Pathogenesis·Dilan R WeerakoonJonathan W Olson
Nov 6, 2015·Virulence·Kshipra ChandrashekharGireesh Rajashekara
Sep 2, 2014·Metabolomics : Official Journal of the Metabolomic Society·Robert M HowlettDavid J Kelly
Jul 27, 2010·Environmental Microbiology·Marie T ThomasBruce M Pearson
Jan 25, 2011·Microbial Biotechnology·Byeonghwa JeonQijing Zhang
Dec 23, 2009·Molecular Microbiology·Lauren E Hartley-TassellVictoria Korolik
Apr 9, 2014·Environmental Microbiology·Anne-Xander van der StelMarc M S M Wösten
Dec 15, 2010·Research in Microbiology·Bachar AlazzamGwennola Ermel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Campylobacteriosis

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.