Amino acid derivative-mediated detoxification and functionalization of dual cure dental restorative material for dental pulp cell mineralization

Biomaterials
Hajime MinamikawaTakahiro Ogawa

Abstract

Current dental restorative materials are only used to fill the defect of hard tissues, such as dentin and enamel, because of their cytotoxicity. Therefore, exposed dental pulp tissues in deep cavities must be first covered by a pulp capping material like calcium hydroxide to form a layer of mineralized tissue. However, this tissue mineralization is based on pathological reaction and triggers long-lasting inflammation, often causing clinical problems. This study tested the ability of N-acetyl cysteine (NAC), amino acid derivative, to reduce cytotoxicity and induce mineralized tissue conductivity in resin-modified glass ionomer (RMGI), a widely used dental restorative material having dual cure mechanism. Rat dental pulp cells were cultured on untreated or NAC-supplemented RMGI. NAC supplementation substantially increased the percentage of viable cells from 46.7 to 73.3% after 24-h incubation. Cell attachment, spreading, proliferative activity, and odontoblast-related gene and protein expressions increased significantly on NAC-supplemented RMGI. The mineralization capability of cells, which was nearly suppressed on untreated RMGI, was induced on NAC-supplemented RMGI. These improved behaviors and functions of dental pulp cells on ...Continue Reading

References

May 1, 1987·Clinical Orthopaedics and Related Research·N A JohansonC S Ranawat
Jan 1, 1985·Archives of Orthopaedic and Trauma Surgery·S B GoodmanC Hunt
Dec 1, 1982·Journal of Oral Pathology·Z HirschfeldJ Sela
Mar 1, 1994·International Endodontic Journal·D Tziafas
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·W R Hume, T M Gerzina
Jul 11, 1998·Respiratory Medicine·A Gillissen, D Nowak
Oct 13, 1998·Journal of Cataract and Refractive Surgery·S SaikaA Yamanaka
Aug 22, 2000·Journal of Biomedical Materials Research·G CiapettiA Pizzoferrato
Oct 13, 2001·Journal of Dental Research·H SchweiklT Spruss
Jan 5, 2002·Physiological Reviews·Wulf Dröge
Oct 16, 2002·Annals of the Rheumatic Diseases·E F Wagner
Feb 18, 2003·Australian Dental Journal·G J MountO F Makinson
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·M ZafarullahM Ahmad
Feb 6, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Michel Goldberg, Anthony J. Smith
Jan 25, 2005·Biomaterials·Helmut SchweiklGottfried Schmalz
Oct 20, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lei SaruwatariTakahiro Ogawa
Jan 3, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·Pedro P C SouzaCarlos A de Souza Costa
Aug 8, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·H SchweiklG Schmalz
Sep 26, 2006·Journal of Dental Research·H SchweiklG Schmalz
Feb 19, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Alexandre M FernandesJosé B Alves
Mar 26, 2008·Journal of Dental Research·M YamadaT Ogawa
Jun 10, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·N KojimaT Ogawa
Jun 10, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·John W Nicholson, Beata Czarnecka
Nov 29, 2008·Cytokine & Growth Factor Reviews·Frédéric BlanchardBénédicte Brounais
Dec 8, 2009·Journal of Dental Research·D W BerzinsH W Roberts
Dec 17, 2009·Cell Host & Microbe·Eric Vivier, Sophie Ugolini

❮ Previous
Next ❯

Citations

May 29, 2013·Biomaterials·Masahiro YamadaTakahiro Ogawa
Jan 9, 2014·Journal of Materials Science. Materials in Medicine·Nattaporn WanachottrakulUreporn Kedjarune-Leggat
Dec 10, 2015·Operative Dentistry·G SchmalzK M Galler
Apr 18, 2015·The Journal of Prosthetic Dentistry·Kaori NakagawaTakahiro Ogawa
Sep 30, 2010·Journal of Biomaterials Applications·Masahiro YamadaTakahiro Ogawa
Dec 3, 2017·Clinical Oral Investigations·Aletéia M M FernandesGottfried Schmalz
Apr 5, 2020·International Journal of Molecular Sciences·Yoshihiko SugitaTakahiro Ogawa
Jun 10, 2020·International Journal of Molecular Sciences·Kosuke HamajimaTakahiro Ogawa
Dec 14, 2017·Scientific Reports·Fabíola CostaM Cristina L Martins
Sep 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lingxin ZhuBin Peng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis