Amino acid metabolism during batch culture of a murine hybridoma, AFP-27.

Cytotechnology
C P MarquisC Harbour

Abstract

This paper presents batch culture data of the murine hybridoma, AFP-27, cultured in conventional basal media and in a nutrient-rich modified version. Expression of antibody was fivefold higher in the enriched formulation, with significant product secretion in the decline phase. Cultures were initiated at conventional inculation densities (1 ∼ 2 × 10(5) viable cells ml(-1)) and high inoculation densities (1.5 ∼ 1.7 × 10(6) viable cells ml(-1)). Amino acid levels have been reported for all cultures, with apparent differences described. Relative levels of intracellular amino acids are also reported, with significant accumulation of proline, glycine and alanine. The results have significance in the design of enriched media which are clearly beneficial for commercial production of antibodies from hybridomas.

References

Apr 1, 1978·Journal of Cellular Physiology·H R ZielkeM Cornblath
Jan 1, 1991·Progress in Biophysics and Molecular Biology·C I Cheeseman
Jan 1, 1988·Metabolism: Clinical and Experimental·M S Ardawi
Jan 1, 1985·The Journal of Membrane Biology·H N Christensen
Sep 1, 1980·Journal of Cellular Physiology·H R ZielkeP T Ozand
Jun 20, 1990·Biotechnology and Bioengineering·K K Frame, W S Hu
Nov 5, 1994·Biotechnology and Bioengineering·S Mercille, B Massie

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Citations

Feb 7, 2008·Bioprocess and Biosystems Engineering·Yuan-Hua LiuJing-Qi Yuan
Jan 9, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Agnes MichalczykMargaret L Ackland
Dec 8, 2011·Molecular Nutrition & Food Research·Dennis MulacHans-Ulrich Humpf

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