Amino acid requirements for growth of Chlamydia pneumoniae in cell cultures: growth enhancement by lysine or methionine depletion.

Journal of Clinical Microbiology
C C Kuo, J T Grayston

Abstract

Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that among 13 amino acids in Eagle minimum essential medium, C. pneumoniae required all amino acids except lysine. A true requirement for arginine, isoleucine, leucine, threonine, and valine could not be determined because depletion of 100% of these amino acids caused cell detachment. Consequently, a requirement for these amino acids was based on 90% depletion. C. trachomatis biovar trachoma required all amino acids except threonine, which was indeterminable. Depletion of 100 and 90% of the lysine and 90 to 70% of the methionine was shown to enhance the growth of C. pneumoniae. This phenomenon was shown to be a property of C. pneumoniae because the effect of lysine and methionine reduction was also demonstrated in another human line, HL cells, and a mouse line, McCoy cells.

References

Mar 1, 1977·Journal of Bacteriology·D K Smith, H H Winkler
Mar 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J T GraystonL A Campbell
Dec 1, 1986·Journal of Clinical Microbiology·C C KuoJ T Grayston
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Dec 1, 1973·FEBS Letters·D F Niven, W A Hamilton
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Citations

Jul 24, 2004·Infection and Immunity·Hesham M Al-YounesThomas F Meyer
Jan 24, 2006·Journal of Bacteriology·Chris S Schaumburg, Ming Tan
Dec 21, 2016·Molecular Microbiology·Adrian MehlitzThomas Rudel
Oct 1, 1995·Clinical Microbiology Reviews·C C KuoJ T Grayston
Mar 1, 1991·Microbiological Reviews·J W Moulder
Jan 29, 2008·Microbial Pathogenesis·Joscha GussmannThomas F Meyer

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