Amino acid sequence and biological activity of a calcitonin-like diuretic hormone (DH31) from Rhodnius prolixus

The Journal of Experimental Biology
Victoria A Te BruggeI Orchard

Abstract

Diuresis in the blood-gorging hemipteran Rhodnius prolixus is under neurohormonal control and involves a variety of processes and tissues. These include ion and water movement across the epithelium of the crop and the Malpighian tubules, and muscle contractions of the crop, hindgut and dorsal vessel, which facilitate mixing of the blood-meal, mixing of the haemolymph, as well as the expulsion of waste. One of the neurohormones that might play a role in this rapid diuresis belongs to the calcitonin-like diuretic hormone (DH(31)) family of insect peptides. Previously we have demonstrated the presence of DH(31)-like peptides in the central nervous system (CNS) and gut of R. prolixus 5th instars. In the present work, a DH(31) from the CNS of 5th instar R. prolixus was isolated using reversed-phase liquid chromatography (RPLC), monitored with an enzyme-linked immunosorbent assay (ELISA) combined with matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, and sequenced using tandem mass spectrometry and Edman degradation. This neuropeptide is the first to be sequenced in R. prolixus and has a sequence identical to that found previously for Dippu-DH(31) from the cockroach Diploptera punctata. In prev...Continue Reading

References

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Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·K FuruyaD A Schooley
Mar 19, 2002·Peptides·V A Te BruggeI Orchard
Aug 20, 2005·The Journal of Experimental Biology·Geoffrey M CoastDavid A Schooley
Feb 17, 2006·The Journal of Experimental Biology·Jean-Paul Paluzzi, Ian Orchard

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Citations

Mar 26, 2013·Insect Biochemistry and Molecular Biology·Hyeogsun Kwon, Patricia V Pietrantonio
Apr 9, 2014·Journal of Molecular Endocrinology·David A LovejoyJon del Castillo
Jul 24, 2012·Insect Biochemistry and Molecular Biology·Meet Zandawala
Dec 10, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Marcos SterkelJorge Rafael Ronderos
May 2, 2009·General and Comparative Endocrinology·Victoria Te BruggeIan Orchard
Jan 13, 2009·Proteomics·Sheila OnsRolando Rivera Pomar
May 22, 2009·Annals of the New York Academy of Sciences·Ian Orchard, Jean-Paul Paluzzi
Oct 21, 2010·Insect Molecular Biology·S OnsR Rivera-Pomar
Oct 22, 2011·Peptides·Reinhard PredelRonald J Nachman
Mar 16, 2021·Frontiers in Physiology·Ana Caroline P GandaraPedro L Oliveira
Jan 20, 2009·The Journal of Experimental Biology·Geoffrey M Coast

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