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Amino acid sequence based PCR primers for amplification of rearranged human heavy and light chain immunoglobulin variable region genes

Journal of Immunological Methods

Feb 27, 1995

M WelschofG Moldenhauer

PMID: 7876568

Abstract

Previously described primers for PCR amplification of variable immunoglobulin (Ig) genes were based on gene sequences. To include the large number of amino acid sequences of antibodies whose DNA has not been sequenced and to ensure a maximal fit to rearranged human Ig variable region ge...read more

Mentioned in this Paper

Homologous Sequences, Amino Acid
Immunoglobulin Light Chains
Immunoglobulin Heavy Chain Subgroup VH-III
Oligonucleotide Primers
Framework Region, Immunoglobulin
Consensus Sequence
Genes, Immunoglobulin
Restriction Mapping
Gene Rearrangement, B-Lymphocyte
Determination, Sequence Homology
Paper Details
References
  • References25
  • Citations47
123
  • References25
  • Citations47
12345

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Amino acid sequence based PCR primers for amplification of rearranged human heavy and light chain immunoglobulin variable region genes

Journal of Immunological Methods

Feb 27, 1995

M WelschofG Moldenhauer

PMID: 7876568

DOI:

Abstract

Previously described primers for PCR amplification of variable immunoglobulin (Ig) genes were based on gene sequences. To include the large number of amino acid sequences of antibodies whose DNA has not been sequenced and to ensure a maximal fit to rearranged human Ig variable region ge...read more

Mentioned in this Paper

Homologous Sequences, Amino Acid
Immunoglobulin Light Chains
Immunoglobulin Heavy Chain Subgroup VH-III
Oligonucleotide Primers
Framework Region, Immunoglobulin
Consensus Sequence
Genes, Immunoglobulin
Restriction Mapping
Gene Rearrangement, B-Lymphocyte
Determination, Sequence Homology

Similar Papers Found In These Feeds

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Immunoglobulin class switching from igm to igg and iga is central to immunity against viruses and requires the activation of b cells by t cells via cd154 and cytokines. Discover the latest research on immunoglobulin class switching here.

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The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody

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M WelschofG Opelz
Immunotechnology : an International Journal of Immunological Engineering

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Paper Details
References
  • References25
  • Citations47
123
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12345
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