Amino acid sequence of nuclease S1 from Aspergillus oryzae

Journal of Biochemistry
A IwamatsuF Sakiyama

Abstract

The amino acid sequence of nuclease S1, a nuclease which cleaves both single-stranded DNA and RNA, from Aspergillus oryzae was determined. Reduced and S-carboxymethylated or S-aminoethylated nuclease S1 was digested with Achromobacter protease I, Staphylococcus aureus V8 protease, or endoproteinase Asp-N. Peptides thus obtained were purified by reverse-phase high-performance liquid chromatography and sequenced, and the complete primary structure was established. Nuclease S1 consists of a single peptide chain of 267 amino acid residues bearing N-glycosylated Asns 92 and 228. Five half-cystine residues are present at positions 25, 72, 80, 85, and 216, and the latter four residues are implicated in the formation of disulfide bonds by analogy with those in nuclease P1. Two short stretches of sequences involving His 60 and His 125 are shown to be identical with those involving active site His 119 in bovine ribonuclease A and active-site His 134 in porcine deoxyribonuclease I, respectively.

Citations

Jan 1, 1995·Critical Reviews in Microbiology·S U Gite, V Shankar
Dec 1, 1992·European Journal of Biochemistry·S Gite, V Shankar
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Karine TriquesAbdelhafid Bendahmane
Feb 15, 2003·FEMS Microbiology Reviews·Neelam A Desai, Vepatu Shankar
Sep 15, 1991·European Journal of Biochemistry·K MaekawaF Sakiyama
Feb 6, 2007·The Journal of Biological Chemistry·Manju B Joshi, Dennis M Dwyer
May 5, 2021·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Feb 19, 2002·International Journal for Parasitology·William F SopwithPaul A Bates
Oct 17, 2002·Analytical Biochemistry·Barbara MouratouJean-Luc Guesdon
Jun 26, 2004·International Journal for Parasitology·S FarajniaF Mahboudi

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