PMID: 9163349Apr 15, 1997Paper

Amino acid sequence of the alpha subunit and computer modelling of the alpha and beta subunits of echicetin from the venom of Echis carinatus (saw-scaled viper)

The Biochemical Journal
J PolgarKenneth J Clemetson

Abstract

Echicetin, a heterodimeric protein from the venom of Echis carinatus, binds to platelet glycoprotein Ib (GPIb) and so inhibits platelet aggregation or agglutination induced by various platelet agonists acting via GPIb. The amino acid sequence of the beta subunit of echicetin has been reported and found to belong to the recently identified snake venom subclass of the C-type lectin protein family. Echicetin alpha and beta subunits were purified. N-terminal sequence analysis provided direct evidence that the protein purified was echicetin. The paper presents the complete amino acid sequence of the alpha subunit and computer models of the alpha and beta subunits. The sequence of alpha echicetin is highly similar to the alpha and beta chains of various heterodimeric and homodimeric C-type lectins. Neither of the fully reduced and alkylated alpha or beta subunits of echicetin inhibited the platelet agglutination induced by von Willebrand factor-ristocetin or alpha-thrombin. Earlier reports about the inhibitory activity of reduced and alkylated echicetin beta subunit might have been due to partial reduction of the protein.

Citations

Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Takashi Morita
May 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Qiumin LuKenneth J Clemetson
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Q LuK J Clemetson
Oct 19, 2001·Oncogene·B A Judd, G A Koretzky
Oct 19, 2001·Oncogene·A Y TsygankovG Swaminathan
Oct 8, 1999·Biochemical and Biophysical Research Communications·C H ChungT F Huang
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·Ayako TaniMotonori Ohno
Sep 15, 1999·Toxicon : Official Journal of the International Society on Toxinology·Y KomoriH Sugihara
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·F S Markland
Mar 20, 1999·Biochimica Et Biophysica Acta·B R JenningsE G Shephard
Dec 9, 2003·Journal of Molecular Biology·Jayasankar JastiT P Singh
Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Alexandre HavtHelena Serra Azul Monteiro
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Tomohisa OgawaMotonori Ohno
Sep 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·Brent JenningsEnid Shephard

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