Amino acid sequence studies of horseradish peroxidase. Amino and carboxyl termini, cyanogen bromide and tryptic fragments, the complete sequence, and some structural characteristics of horseradish peroxidase C
Horseradish peroxidase C dominates quantitatively among the isoperoxidases of horseradish root and has an isoelectric point close to 9. It consists of a hemin prosthetic group, 2 Ca2+ and 308 amino acid residues, including 4 disulfide bridges, in a single polypeptide chain that carries 8 neutral carbohydrate side-chains. The molecular weight of the polypeptide chain is 33890. Assuming an average carbohydrate composition of (GlcNAc)2, Man3, Fuc, Xyl for each carbohydrate chain, the molecular weight of native horseradish peroxidase C is close to 44 000. Cyanogen bromide fragments of reduced and carboxymethylated apo-peroxidase were purified by a combination of gel filtration and isoelectric focusing in urea, and cystine-containing tryptic fragments of apo-peroxidase were purified by gel filtration followed by disulfide cleavage and rechromatography at the initial conditions. The present paper discusses (a) isoelectric points and charge distribution within the native protein, the apoprotein and the cyanogen bromide fragments, (b) a buried pyrrolidonecarboxylyl amino terminus, (c) heterogeneity at the carboxyl terminus, and (d) a possible domain structure, likely from partial tryptic digestion.
Amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip
Similarities and differences of five peroxidases from turnip and horseradish. Peptide mapping studies on glycoproteins
Amino acid sequences studies of horseradish peroxidase. Tryptic glycopeptide containing two histidine residues and a disulfide bridge
Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits
Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression
Cellular location of peroxidase isoenzymes in leaf tissue of Petunia and their affinity for Concanavalin A-Sepharose
Competition between glycoprotein hormones and horseradish peroxidase for mannose-specific binding sites in cells of endocrine organs
The inactivation of horseradish peroxidase isoenzyme A2 by hydrogen peroxide: an example of partial resistance due to the formation of a stable enzyme intermediate
Investigating the structural and functional effects of mutating Asn glycosylation sites of horseradish peroxidase to Asp
Effect of lentinan on pinocytosis in mouse peritoneal macrophages and the murine macrophage cell line C4M phi in vitro
Characterization of genes expressed in mesophyll protoplasts of Nicotiana sylvestris before the re-initiation of the DNA replicational activity
Haem-linked interactions in horseradish peroxidase revealed by spectroscopic analysis of the Phe-221-->Met mutant
Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression
Annexin-like protein from Arabidopsis thaliana rescues delta oxyR mutant of Escherichia coli from H2O2 stress
Transport into and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase
Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalyzed by a 40-kilodalton peroxidase
Elimination of Differences in the Mobility of Flax Isoperoxidases on PAGE by Digestion with alpha-Mannosidase
Covalent structure of turnip peroxidase 7. Cyanogen bromide fragments, complete structure and comparison to horseradish peroxidase C
Plant peroxidases. Their primary, secondary and tertiary structures, and relation to cytochrome c peroxidase
Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F cannot release glycans with fucose attached alpha 1----3 to the asparagine-linked N-acetylglucosamine residue
A step towards understanding the folding mechanism of horseradish peroxidase. Tryptophan fluorescence and circular dichroism equilibrium studies
Horseradish peroxidase isozyme C. A comparative study of native and recombinant enzyme produced by E. coli transformants
Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness
A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris
A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay
Oxidation of ferulic acid by Momordica charantia peroxidase and related anti-inflammation activity changes
Enzyme-labeled antigen method: histochemical detection of antigen-specific antibody-producing cells in tissue sections of rats immunized with horseradish peroxidase, ovalbumin, or keyhole limpet hemocyanin
Oxidation of 4-tert-butylcatechol and dopamine by hydrogen peroxide catalysed by horseradish peroxidase
Modification of lysine residues of horseradish peroxidase and its effect on stability and structure of the enzyme
Molecular cloning and partial characterization of a peroxidase gene expressed in the roots of Portulaca oleracea cv., one potentially useful in the remediation of phenolic pollutants
The biological effects of vanadyl curcumin and vanadyl diacetylcurcumin complexes: the effect on structure, function and oxidative stability of the peroxidase enzyme, antibacterial activity and cytotoxic effect
Catalytic role of traditional enzymes for biosynthesis of biogenic metallic nanoparticles: a mini-review
Nano reengineering of horseradish peroxidase with dendritic macromolecules for stability enhancement
Purification, biochemical characterization and cloning of a new cationic peroxidase isoenzyme from artichoke
A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles
High-efficiency secretory production of peroxidase C1a using vesicular transport engineering in transgenic tobacco
Genetically engineered horseradish peroxidase for facilitated purification from baculovirus cultures by cation-exchange chromatography
Isolation and characterization of the first putative peroxidase gene from oilseed rape (Brassica napus) which is highly homologous to HRPC
Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris
An updated view on horseradish peroxidases: recombinant production and biotechnological applications
How modification of accessible lysines to phenylalanine modulates the structural and functional properties of horseradish peroxidase: a simulation study
Structural stabilization and functional improvement of horseradish peroxidase upon modification of accessible lysines: experiments and simulation
Mössbauer and electron paramagnetic resonance studies of horseradish peroxidase and its catalytic intermediates
Activity of the C-terminal-dependent vacuolar sorting signal of horseradish peroxidase C1a is enhanced by its secondary structure
A nuclear Overhauser effect investigation of the molecular and electronic structure of the heme crevice in lactoperoxidase
Oxidation-reduction potentials and ionization states of extracellular peroxidases from the lignin-degrading fungus Phanerochaete chrysosporium
Structure of the active site of lignin peroxidase isozyme H2: native enzyme, compound III, and reduced form
Intermediate spin-states in one-electron reduction of oxygen-hemoprotein complexes at low temperature
The role of the mannose/N-acetylglucosamine receptor in the pinocytosis of horseradish peroxidase by mouse peritoneal macrophages
Molecular diffusion within sol-gel derived matrices viewed via fluorescence recovery after photobleaching
Characterization of oligosaccharide moieties of glycopeptides by microwave-assisted partial acid hydrolysis and mass spectrometry
Refinement of 3D models of horseradish peroxidase isoenzyme C: predictions of 2D NMR assignments and substrate binding sites
Role of arginine 38 in horseradish peroxidase. A critical residue for substrate binding and catalysis
Electrochemical detection of extracellular hydrogen peroxide released from RAW 264.7 murine macrophage cells based on horseradish peroxidase-hydroxyapatite nanohybrids
Comparison of three palm tree peroxidases expressed by Escherichia coli: uniqueness of African oil palm peroxidase
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