PMID: 11343790May 10, 2001Paper

Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom

Biochimica Et Biophysica Acta
A GasmiM El Ayeb

Abstract

A heterodimeric disintegrin designed as lebein was isolated from crude Vipera lebetina venom using gel filtration, anion and cation exchange chromatographies on FPLC. The amino acid sequence of each subunit determined by Edman degradation contains 64 residues with ten half-cystines and an RGD site at the C-terminal part of the molecule. The molecular mass of native lebein determined by mass spectrometry was found to be 14083.4 Da and those of alpha and beta subunits were 6992.05 and 7117.62, respectively. These value are in good agreement with those calculated from the sequences. This protein strongly inhibits ADP induced platelet aggregation on human platelet rich plasma with IC(50)=160 nM. Sequences of this protein subunits displayed significant sequence similarities with many other monomeric and dimeric disintegrins reported from snake venoms. We identified an amino acid residue (N) in the hairpin loop of both subunits (CNRARGDDMNDYC) which is different from all other reported motifs of disintegrins and this subtle difference may contribute to the distinct affinities and selectivities of this class of proteins.

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Citations

Feb 5, 2003·Biochemical and Biophysical Research Communications·Shao Hui WangXiang Fu Wu
Mar 20, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Heiki VijaJüri Siigur
Sep 15, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xing-Ding ZhouYu-Liang Xiong
Apr 14, 2016·Biologicals : Journal of the International Association of Biological Standardization·Sara LucenaElda E Sánchez
Feb 15, 2007·Insect Molecular Biology·F E Cázares-RagaF de la Cruz Hernández-Hernández
Oct 27, 2018·Current Drug Targets·Pedro Henrique Souza CesarSilvana Marcussi
Apr 30, 2003·The Journal of Biological Chemistry·Johannes A EbleUlrike Mayer
Jul 12, 2016·Toxins·Manel B HammoudaKhadija Essafi-Benkhadir
Feb 23, 2020·Journal of Proteomics·Julien GiribaldiSebastien Dutertre
Aug 2, 2003·Archives of Biochemistry and Biophysics·M R CominettiH S Selistre-de-Araujo

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