PMID: 8955402Dec 1, 1996Paper

Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae

Journal of Bacteriology
M B WrightR F Gaber

Abstract

Selection for the ability of Saccharomyces cerevisiae cells to take up histidinol, the biosynthetic precursor to histidine, results in dominant mutations at HOL1. The DNA sequence of HOL1 was determined, and it predicts a 65-kDa protein related to the major facilitator family (drug resistance subfamily) of putative transport proteins. Two classes of mutations were obtained: (i) those that altered the coding region of HOL1, conferring the ability to take up histidinol; and (ii) cis-acting mutations (selected in a mutant HOL1-1 background) that increased expression of the Hol1 protein. The ability to transport histidinol and other cations was conferred by single amino acid substitutions at any of three sites located within putative membrane-spanning domains of the transporter. These mutations resulted in the conversion of a small hydrophobic amino acid codon to a phenylalanine codon. Selection for spontaneous mutations that increase histidinol uptake by such HOL1 mutants resulted in mutations that abolish the putative start codon of a six-codon open reading frame located approximately 171 nucleotides downstream of the transcription initiation site and 213 nucleotides upstream of the coding region of HOL1. This single small upstre...Continue Reading

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Citations

Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marie OsterbergGunnar von Heijne
Jun 12, 2009·Annual Review of Microbiology·Heather M HoodMatthew S Sachs
Jun 10, 2015·The Journal of Biological Chemistry·Kathryn D SmithScott A Strobel
May 21, 1998·Fungal Genetics and Biology : FG & B·M S Sachs
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·J E McCarthy

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