Amino acid transporter expression and localisation studies in pea (Pisum sativum)

Functional Plant Biology : FPB
Mechthild TegederJohn W Patrick

Abstract

Expression of the amino acid permeases PsAAP1 and PsAAP2 was analysed in developing pea (Pisum sativum L.) plants. Both transporters were expressed in seed coats and cotyledon epidermal transfer cells and storage parenchyma cells. AAP expression is developmentally regulated and coincides with the onset of storage protein synthesis. Nitrogen was shown to induce AAP expression and AAP transcript levels were upregulated during the photoperiod. Analysis of Arabidopsis thaliana AAP1 promoter activity in pea, using promoter-β-glucuronidase (promotor-GUS) studies, revealed targeting of GUS to seed coats and cotyledon epidermal transfer cells. Expression was found in the nutritious endosperm during the early stages of seed development, whereas GUS staining in embryos was detected from the heart stage onward. In addition, AAP1 expression was observed in the phloem throughout the plant. This finding equally applied to PsAAP1 expression as shown by in situ mRNA hybridisation, which also demonstrated that PsAAP1 expression was localised to companion cells. Overall, PsAAP1 expression patterns and cellular localisation point to a function of the transporter in phloem loading of amino acids for translocation to sinks and in seed loading for d...Continue Reading

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Citations

Oct 30, 2014·The Plant Journal : for Cell and Molecular Biology·Lizhi ZhangMechthild Tegeder
Aug 17, 2018·Journal of Experimental Botany·Matthew G GarneauMechthild Tegeder
Nov 10, 2017·The New Phytologist·Mechthild Tegeder, Céline Masclaux-Daubresse
Aug 13, 2014·Journal of Experimental Botany·Réjane Pratelli, Guillaume Pilot
Feb 21, 2021·Environmental Science and Pollution Research International·Jun-Feng WangZhi-Min Xu
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder
Mar 27, 2016·Plant Physiology·James P Santiago, Mechthild Tegeder
Aug 21, 2021·Journal of Plant Physiology·Aart J E van Bel

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