Amino Acid Variation at VP1-145 of Enterovirus 71 Determines Attachment Receptor Usage and Neurovirulence in Human Scavenger Receptor B2 Transgenic Mice

Journal of Virology
Kyousuke KobayashiSatoshi Koike

Abstract

Infection by enterovirus 71 (EV71) is affected by cell surface receptors, including the human scavenger receptor B2 (hSCARB2), which are required for viral uncoating, and attachment receptors, such are heparan sulfate (HS), which bind virus but do not support uncoating. Amino acid residue 145 of the capsid protein VP1 affects viral binding to HS and virulence in mice. However, the contribution of this amino acid to pathogenicity in humans is not known. We produced EV71 having glycine (VP1-145G) or glutamic acid (VP1-145E) at position 145. VP1-145G, but not VP1-145E, enhanced viral infection in cell culture in an HS-dependent manner. However, VP1-145G virus showed an attenuated phenotype in wild-type suckling mice and in a transgenic mouse model expressing hSCARB2, while VP1-145E virus showed a virulent phenotype in both models. Thus, the HS-binding property and in vivo virulence are negatively correlated. Immunohistochemical analyses showed that HS is highly expressed in vascular endothelial cells and some other cell types where hSCARB2 is expressed at low or undetectable levels. VP1-145G virus bound to tissue homogenate of both hSCARB2 transgenic and nontransgenic mice in vitro, and the viral titer was reduced in the bloodstre...Continue Reading

References

Sep 25, 1999·The New England Journal of Medicine·M HoS R Shih
Oct 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L G ChanL J Anderson
Mar 16, 2005·Journal of Virology·Miki Ida-HosonumaSatoshi Koike
Jun 19, 2008·The Journal of General Virology·Beng Hooi ChuaPeter C McMinn
Jun 23, 2009·Nature Medicine·Seiya YamayoshiSatoshi Koike
Jun 23, 2009·Nature Medicine·Yorihiro NishimuraHiroyuki Shimizu
Jun 22, 2011·Cold Spring Harbor Perspectives in Biology·Stephane SarrazinJeffrey D Esko
Nov 1, 2011·PloS One·Renqing LiYumei Wang
Mar 23, 2012·Journal of Virology·Seiya YamayoshiSatoshi Koike
Dec 1, 2012·Emerging Infectious Diseases·Truong Huu KhanhH Rogier van Doorn
Jan 11, 2013·Journal of Virology·Seiya YamayoshiSatoshi Koike
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ken FujiiSatoshi Koike
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Mar 14, 2014·Journal of Virology·Ning DuPo Tien
Feb 13, 2015·Journal of Virology·Pei-Yi SuChuan-Fa Chang

❮ Previous
Next ❯

Citations

Aug 2, 2019·Future Microbiology·Xingjian WenAnchun Cheng
Jan 12, 2020·Journal of Biomedical Science·Kyousuke Kobayashi, Satoshi Koike
Dec 16, 2018·Virologica Sinica·Huiqiang Wang, Yuhuan Li
Jan 17, 2021·Journal of Biomedical Science·Mohd Ishtiaq AnasirChit Laa Poh
Jan 28, 2021·Viruses·Christine E Peters, Jan E Carette
Feb 2, 2021·Frontiers in Microbiology·Chih-Shin ChangChiaho Shih
Nov 14, 2020·Expert Review of Anti-infective Therapy·Han Kang TeeYoke Fun Chan
Mar 23, 2021·Emerging Microbes & Infections·Pei Yi AngSylvie Alonso
Jul 3, 2021·International Journal of Molecular Sciences·Valeria De PasqualeLuigi Michele Pavone
Aug 29, 2021·Viruses·Kyousuke Kobayashi, Satoshi Koike
Sep 30, 2021·Journal of Nanobiotechnology·Xianliang KeYuan Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.