PMID: 589477Dec 16, 1977

Amino acids in the synaptic vesicle fraction from calf brain: content, uptake and metabolism

Brain Research
P LähdesmäkiR Winter


A synaptic vesicle fraction was prepared from calf brain cortex, containing 10 identified amino acids and two unidentified ninhydrin-positive compounds, one of which is apparently a peptide. The most plentiful amino acids were taurine (1.8 nmol/g original tissue), glutamic acid (1.8), serine (0.9), aspartic acid (0.8) and GABA (0.8); the others identified were cysteic acid (or cysteinesulphinic acid), glutamine, alanine, glycine and lysine. The unknown peptide occurred in a high concentration (about 16 alanine equivalents/g), and contained mainly aspartic acid and serine. Cysteic acid (or cysteinesulphinic acid) also occurred in relatively high amounts, but its peak contained acid-labile impurities. The influx of [14C]glutamate into the vesicles took place by means of non-saturable migration, while two saturable systems having very similar properties were dominant only at low glutamate concentrations. Influx constants for these quantitatively low uptake systems were Km, 34 and 92 micrometer, and Vmax, 33 and 49 nmol/min/g obtained by v versus v/S plot. Almost the same values were also obtained by a 1/v versus 1/S plot. GAD and GABA-T activities in the vesicles were only 1/200th of those in the synaptosomes.


Jun 1, 1973·Die Naturwissenschaften·V P Whittaker
Oct 5, 1973·Biochimica Et Biophysica Acta·I G MorganG Gombos
Feb 2, 1973·Science·S BerlW J Nicklas
Aug 1, 1973·Journal of Neurochemistry·R H OsborneD G Jones
Aug 1, 1973·Journal of Neurochemistry·J S De Belleroche, H F Bradford
Feb 1, 1964·The Biochemical Journal·V P WhittakerR J Kirkland
Oct 1, 1966·Journal of Neurochemistry·R BalázsJ R Harwood
Jan 1, 1966·The Biochemical Journal·J L Mangan, V P Whittaker


Nov 1, 1995·Behavioural Brain Research·M Fillenz
Jan 1, 1982·Neurochemistry International·J Gomez, M Fillenz
Oct 30, 1990·Neuroscience Letters·G A TiggesG R Dutton
Jan 1, 1979·Neuroscience·H Zimmermann
Nov 1, 1979·Journal of Neurochemistry·K H Tachiki, C F Baxter
Feb 1, 1979·Acta Physiologica Scandinavica·A KarppinenP Lähdesmäki
Apr 1, 1990·Journal of Neurochemistry·H ChristensenF Fonnum
Mar 11, 2004·The European Journal of Neuroscience·Karina TuzHerminia Pasantes-Morales
Apr 1, 1988·Journal of Neurochemistry·E M Fykse, F Fonnum
Mar 1, 1985·Journal of Neurochemistry·K M MarnelaP Lähdesmäki
Dec 1, 1984·Journal of Neurochemistry·K M MarnelaP Lähdesmäki
May 1, 1984·Journal of Neurosurgery·B K Siesjö

Related Concepts

4-Aminobutyrate aminotransferase
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Bos indicus
Gamma-Aminobutyric Acid, Zinc Salt (2:1)
Glutamate Decarboxylase
Glutamic Acids
Nerve Tissue Proteins
Synaptic Vesicles

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.